A fluorogenic substrate for β-galactosidase
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4-Methylumbelliferyl-β-D-Galactoside

Item No. 33920

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
7-(β-D-galactopyranosyloxy)-4-methyl-2H-1-benzopyran-2-one
CAS Number
6160-78-7
Synonyms
  • 4-Methylumbelliferyl-β-D-Galactopyranoside
Molecular Formula
C16H18O8
Formula Weight
338.3
Purity
≥98%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: slightly solubleEthanol: slightly solubleMethanol: slightly soluble
λmax
319 nm
SMILES
CC(C1=CC=C(O[C@@H]2O[C@@H]([C@@H]([C@@H]([C@H]2O)O)O)CO)C=C1O3)=CC3=O
InChi Code
InChI=1S/C16H18O8/c1-7-4-12(18)23-10-5-8(2-3-9(7)10)22-16-15(21)14(20)13(19)11(6-17)24-16/h2-5,11,13-17,19-21H,6H2,1H3/t11-,13+,14+,15-,16-/m1/s1
InChi Key
YUDPTGPSBJVHCN-DZQJYWQESA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    250 mg

    $36

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    500 mg

    $55

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    1 g

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    5 g

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    Product Description

    4-Methylumbelliferyl-β-D-galactoside (MUG) is a fluorogenic substrate for β-galactosidase (β-gal).1 Hydrolysis of MUG by β-gal releases the fluorescent moiety 4-MU, which displays a pH-dependent excitation maximum of 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively, and an emission maximum between 445-454 nm.2 MUG has been used to detect β-gal activity in intact bacteria, yeast, and mammalian cells.1

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Vidal-Aroca, F., Giannattasio, M., Brunelli, E., et al. One-step high-throughput assay for quantitative detection of β-galactosidase activity in intact Gram-negative bacteria, yeast, and mammalian cells. Biotechniques 40(4), 433-434 (2006).

    2. Zhi, H., Wang, J., Wang, S., et al. Fluorescent properties of hymecromone and fluorimetric analysis of hymecromone in compound dantong capsule. J. Spectrosc. 147128 (2013).