Pure recombinant protein
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Measurement and Detection Target(s)
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YTHDF2 (human, recombinant)

Item No. 26345

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • CAHL
  • CLL-associated Antigen KW-14
  • HGRG8
  • High-Glucose-Regulated Protein 8
  • Renal Carcinoma Antigen NY-REN-2
  • YTH Domain-containing Family Member 2
  • YTH N6-Methyladenosine RNA Binding Protein 2 (358-502; YTH domain)
Purity
≥90% estimated by SDS-PAGE
Source
Recombinant N-terminal GST-tagged YTHDF2 purified from E. coli
Amino Acids
358-502
MW
43.24 kDa
Formulation (Request formulation change)
50 mM HEPES, pH 7.5, with 150 mM sodium chloride and 10% glycerol
UniProt Accession №
Q9Y5A9
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $528

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    Product Description

    YTH-Domain family member 2 (YTHDF2) is a reader protein that binds to N6-methyladenosine (m6A) to regulate mRNA degradation.1,2 YTHDF2 binds to m6A sites in the 3'-UTR of mRNA through its C-terminal YTH domain and recruits the CCR4-NOT deadenylase complex via its N-terminus to accelerate mRNA localization to decay sites, such as P-bodies, and promote subsequent degradation.1,2,3 Ythdf2 knockout results in a build-up of maternal mRNA in oocytes that induces female-specific infertility in mice.4 Conditional knockout of Ythdf2 increases the number of hematopoietic stem cells (HSCs) without shifting lineage differentiation or inducing malignancies.5 It also increases ex vivo expansion of human umbilical cord HSCs. This product is the C-terminus of YTHDF2 corresponding to residues 358-502 of the human full-length sequence.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Du, H., Zhao, Y., He, J., et al. YTHDF2 destabilizes m6A-containing RNA through direct recruitment of the CCR4-NOT deadenylase complex. Nat. Commun. 7:12626, (2016).

    2. Zhong, L., Liao, D., Zhang, M., et al. YTHDF2 suppresses cell proliferation and growth via destabilizing the EGFR mRNA in hepatocellular carcinoma. Cancer Lett. 442, 252-261 (2019).

    3. Wang, X., Lu, Z., Gomez, A., et al. N6-Methyladenosine-dependent regulation of messenger RNA stability. Nature 505(7481), 117-120 (2014).

    4. Ivanova, I., Much, C., Di Giacomo, M., et al. The RNA m6A reader YTHDF2 is essential for the post-transcriptional regulation of the maternal transcriptome and oocyte competence. Mol. Cell 67(6), 1059-1067 (2017).

    5. Li, Z., Qian, P., Shao, W., et al. Suppression of m6A reader Ythdf2 promotes hematopoietic stem cell expansion. Cell Res. 28(9), 904-917 (2018).