For immunochemical detection of YTHDF2
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Measurement and Detection Tool(s)
26345YTHDF2 (human, recombinant)
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YTHDF2 Polyclonal Antibody

Item No. 27910

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • CLL-associated Antigen KW-14
  • HGRG8
  • High-glucose-regulated Protein 8
  • Renal Carcinoma Antigen NY-REN-2
  • YTH Domain-containing Family Protein 2
Immunogen
Full-length recombinant human YTHDF2 protein
MW
63 kD
Formulation (Request formulation change)
500 µg of protein A-purified polyclonal antibody
Storage Buffer
PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide
Host
Rabbit
Applications
IF, IHC, and WB
Species Reactivity
(+) Human; other species not tested
UniProt Accession №
Q9Y5A9
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    500 µl

    $347

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    Product Description

    YTH-Domain family member 2 (YTHDF2) is a reader protein that binds to N6-methyladenosine (m6A) to regulate mRNA degradation.1,2 YTHDF2 binds to m6A sites in the 3'-UTR of mRNA through its C-terminal YTH domain and recruits the CCR4-NOT deadenylase complex via its N-terminus to accelerate mRNA localization to decay sites, such as P-bodies, and promote subsequent degradation.1,2,3 Ythdf2 knockout results in a build-up of maternal mRNA in oocytes that induces female-specific infertility in mice.4 Conditional knockout of Ythdf2 increases the number of hematopoietic stem cells (HSCs) without shifting lineage differentiation or inducing malignancies.5 It also increases ex vivo expansion of human umbilical cord HSCs. Cayman's YTHDF2 Polyclonal Antibody can be used for immunofluorescence, immunohistochemistry, and Western blot applications. The antibody recognizes YTHDF2 at 63 kDa from human samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Du, H., Zhao, Y., He, J., et al. YTHDF2 destabilizes m6A-containing RNA through direct recruitment of the CCR4-NOT deadenylase complex. Nat. Commun. 7:12626, (2016).

    2. Zhong, L., Liao, D., Zhang, M., et al. YTHDF2 suppresses cell proliferation and growth via destabilizing the EGFR mRNA in hepatocellular carcinoma. Cancer Lett. 442, 252-261 (2019).

    3. Wang, X., Lu, Z., Gomez, A., et al. N6-Methyladenosine-dependent regulation of messenger RNA stability. Nature 505(7481), 117-120 (2014).

    4. Ivanova, I., Much, C., Di Giacomo, M., et al. The RNA m6A reader YTHDF2 is essential for the post-transcriptional regulation of the maternal transcriptome and oocyte competence. Mol. Cell 67(6), 1059-1067 (2017).

    5. Li, Z., Qian, P., Shao, W., et al. Suppression of m6A reader Ythdf2 promotes hematopoietic stem cell expansion. Cell Res. 28(9), 904-917 (2018).