GS-441524

GS-441524 is an antiviral nucleoside analog and a metabolite of remdesivir (Item No. 30354).¹ Upon entry into cells, GS-441524 is metabolized to an active triphosphate form that induces RNA chain termination and inhibits viral polymerases. It is active against Middle East respiratory syndrome coronavirus (MERS-CoV) and severe acute respiratory syndrome CoV (SARS-CoV) in infected primary human airway epithelial (HAE) cells (EC₅₀s = 0.86 and 0.18 µM, respectively). GS-441524 reduces viral plaque formation in SARS-CoV-2-infected Vero E6 and Calu-3 2B4 cells (EC₅₀s = 0.42 and 0.62 mM, respectively).² It is cytoprotective against hepatitis C virus (HCV), yellow fever virus (YFV), dengue virus type 2 (DENV-2), influenza A, and parainfluenza 3 in cell-based assays (EC₅₀s = 4.1, 11, 9.46, 27.9, and 1.71 µM, respectively).³

WARNING This product is not for human or veterinary use.

1. Agostini, M.L., Andres, E.L., Sims, A.C., et al. Coronavirus susceptibility to the antiviral remdesivir (GS-5734) is mediated by the viral polymerase and the proofreading exoribonuclease. mBio 9(2), e00221-00218 (2018).

2. Pruijssers, A.J., George, A.S., Schäfer, A., et al. Remdesivir inhibits SARS-CoV-2 in human lung cells and chimeric SARS-CoV expressing the SARS-CoV-2 RNA polymerase in mice. Cell Rep. 32(3), 107940 (2020).

3. Cho, A., Saunders, O.L., Butler, T., et al. Synthesis and antiviral activity of a series of 1'-substituted 4-aza-7,9-dideazaadenosine C-nucleosides. Bioorg. Med. Chem. 22(8), 2705-2707 (2012).