IFN-γ (mouse, recombinant)

Interferon γ (IFN-γ) is a cytokine and type II interferon with roles in innate and adaptive immunity.¹ It is initially produced by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response to pathogens, and then by CD4⁺ T helper (Th1) cells and CD8⁺ cytotoxic effector T cells as adaptive antigen-specific immunity is developed.^1,2 IFN-γ production is induced in response to pathogen-induced secretion of IL-12 and IL-18 by macrophages and is inhibited by IL-4, IL-10, TGF-β, and various glucocorticoids. It facilitates amplification of antigen presentation, the production of reactive oxygen species (ROS), and the induction of antiviral and antibacterial responses by binding to the interferon γ receptor extracellular domain IFNGR1 (Item No. 32006) to induce signal transduction and gene transcription.^1,3 In addition to its role in infectious disease, IFN-γ enhances the antigenicity of tumor cells, making them more susceptible to IFN-γ-stimulated immune effector cells.⁴ It induces tumor cell apoptosis and necroptosis and inhibits tumor angiogenesis in vitro and in vivo. However, IFN-γ-induced removal of highly antigenic tumor cells can leave behind a population with reduced immunogenicity that can evade immune detection and enhance metastasis. Knockdown of Ifnγ improves insulin resistance in a mouse model of high-fat diet-induced obesity and increases susceptibility to various pathogens in mice.^1,5 Cayman's IFN-γ (mouse, recombinant) protein can be used for ELISA and cell-based assay applications. This protein consists of 133 amino acids, has a calculated molecular weight of 15.5 kDa, and a predicted N-terminus of His23 after signal peptide cleavage.

WARNING This product is not for human or veterinary use.

1. Schroder, K., Hertzog, P.J., Ravasi, T., et al. Interferon-γ: An overview of signals, mechanisms and functions. J. Leukoc. Biol. 75(2), 163-189 (2004).

2. Schoenborn, J.R., and Wilson, C.B. Regulation of interferon-γ during innate and adaptive immune responses. Adv. Immunol. 96, 41-101 (2007).

3. Kak, G., Raza, M., and Tiwari, B.K. Interferon-gamma (IFN-γ): Exploring its implications in infectious diseases. Biomol Concepts 9(1), 64-79 (2018).

4. Zaidi, M.R. The interferon-gamma paradox in cancer. J. Interferon. Cytokine Res. 39(1), 30-38 (2019).

5. Ivashkiv, L.B. IFNγ: Signalling, epigenetics and roles in immunity, metabolism disease and cancer immunotherapy. Nat. Rev. Immunol. 18(9), 545-558 (2018).