Histone H4R3Me1 Monoclonal Antibody (RM195)

Histone H4 is one of four core histone proteins that are involved in the organization of DNA into chromatin.¹ Histones are globular proteins with unstructured N-terminal tails and are subject to a variety of post-translational modifications, such as methylation, acetylation, phosphorylation, and citrullination, that can influence chromatin structure and regulate gene transcription.^1,2 Histone H4 can be monomethylated at arginine 3 (H4R3Me1) by protein arginine methyltransferase 1 (PRMT1), PRMT5, and PRMT6.³ Retinal levels of H4R3Me1 are reduced in a rat model of diabetes induced by streptozotocin (STZ; Item No. 13104) compared with non-diabetic controls.⁴ Cayman's Histone H4R3Me1 Monoclonal Antibody (RM195) can be used for ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications.

WARNING This product is not for human or veterinary use.

1. Wang, Y., Li, M., Stadler, S., et al. Histone hypercitrullination mediates chromatin decondensation and neutrophil extracellular trap formation. J. Cell Biol. 184(2), 205-213 (2009).

2. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

3. Cui, J.Y., Fu, Z.D., and Dempsey, J. The role of histone methylation and methyltransferases in gene regulation. Toxicoepigenetics Core Principles and Applications 31-84 (2018).

4. Wang, W., Sidoli, S., Zhang, W., et al. Abnormal levels of histone methylation in the retinas of diabetic rats are reversed by minocycline treatment. Sci. Rep. 7, 45103 (2017).