Histone H4K20Ac Monoclonal Antibody (RM205)

Histone H4 is one of four core histone proteins that are involved in the organization of DNA into chromatin.¹ Histones are globular proteins with unstructured N-terminal tails and are subject to a variety of post-translational modifications, such as methylation, acetylation, phosphorylation, and citrullination, that can influence chromatin structure and regulate gene transcription.^1,2 Acetylation of histone H4 at lysine 20 (H4K20Ac) is associated with gene repression.³ It is enriched near the transcription start sites of minimally expressed genes and, to a lesser extent, in the gene body regions of highly expressed genes in HeLa-S3 cells. H4K20Ac can be recognized and bound by the bromodomain of the histone acetyltransferase CREB-binding protein (CBP), as well as by the second bromodomain of bromodomain-containing protein 4 (BRD4).⁴ Cayman’s Histone H4K20Ac Monoclonal Antibody (RM205) can be used for chromatin immunoprecipitation (ChIP), ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications.

WARNING This product is not for human or veterinary use.

1. Wang, Y., Li, M., Stadler, S., et al. Histone hypercitrullination mediates chromatin decondensation and neutrophil extracellular trap formation. J. Cell Biol. 184(2), 205-213 (2009).

2. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

3. Kaimori, J.-Y., Maehara, K., Hayashi-Takanaka, Y., et al. Histone H4 lysine 20 acetylation is associated with gene repression in human cells. Sci. Rep. 6, 24318 (2016).

4. Josling, G.A., Selvarajah, S.A., Petter, M., et al. The role of bromodomain proteins in regulating gene expression. Genes (Basel) 3(2), 320-343 (2012).