Polyethylene Glycol Rabbit Monoclonal Antibody (Clone RM105)

Polyethylene glycols (PEGs) are synthetic and hydrophilic polymers.^1,2 They are linear or branched and contain a reactive end group, such as acrylate, methacrylate, dibenzocyclooctynol, or vinyl sulfonate, for covalent attachment to macromolecules or linkers. The opposite end group of PEGs is commonly a methyl group (methoxy PEG), however, hydroxy, amino, butoxy, and tert-butoxy end groups have also been used.¹ PEGs are non-toxic and are commonly used to prolong the in vivo circulation time of pharmaceutical agents.² Free PEGs are non-immunogenic but become immunogenic when conjugated to a drug delivery nanosystem (DDS) or a macromolecule.¹ Immunogenicity of PEGs varies based on polymer length and branching, end group composition, and chemical nature of the PEG acceptor structure. Cayman’s Polyethylene Glycol Rabbit Monoclonal Antibody (Clone RM105) can be used for ELISA, immunohistochemistry (IHC), and Western blot (WB) applications. The antibody recognizes PEGs containing a methoxy end group.

WARNING This product is not for human or veterinary use.

1. Kozma, G.T., Shimizu, T., Ishida, T., et al. Anti-PEG antibodies: Properties, formation, testing and role in adverse immune reactions to PEGylated nano-biopharmaceuticals. Adv. Drug Deliv. Rev. 154-155, 163-175 (2020).

2. Zhang, Z., Zhang, Y., Song, S., et al. Recent advances in the bioanalytical methods of polyethylene glycols and PEGylated pharmaceuticals. J. Sep. Sci. 43(9-10), 1978-1997 (2020).