BSA-PEG(2000)

Polyethylene glycols (PEGs) are synthetic and hydrophilic polymers.^1,2 They are linear or branched and contain a reactive end group, such as acrylate, methacrylate, dibenzocyclooctynol, or vinyl sulfonate, for covalent attachment to macromolecules or linkers. The opposite end group of PEGs is commonly a methyl group (methoxy PEG), however, hydroxy, amino, butoxy, and tert-butoxy end groups have also been used.¹ PEGs are non-toxic and are commonly used to prolong the in vivo circulation time of pharmaceutical agents, and PEGylated BSA has been used as a drug carrier in vitro.^2,3,4 Free PEGs are non-immunogenic but become immunogenic when conjugated to a drug delivery nanosystem (DDS) or a macromolecule.¹ Immunogenicity of PEGs varies based on polymer length and branching, end group composition, and chemical nature of the PEG acceptor structure. Cayman’s BSA-PEG(2000) protein is composed of methoxy-PEG(2000)-NHS ester and BSA at an approximately 10:1 molar ratio of methoxy-PEG(2000)-NHS ester:BSA. This product can be used as a positive control in ELISA and Western blot (WB) experiments using Cayman's Polyethylene Glycol Rabbit Monoclonal Antibody (Clone RM105) (Item No. 32180) and Polyethylene Glycol Rabbit Monoclonal Antibody - Biotinylated (Clone RM105) (Item No. 32381).

WARNING This product is not for human or veterinary use.

1. Kozma, G.T., Shimizu, T., Ishida, T., et al. Anti-PEG antibodies: Properties, formation, testing and role in adverse immune reactions to PEGylated nano-biopharmaceuticals. Adv. Drug Deliv. Rev. 154-155, 163-175 (2020).

2. Zhang, Z., Zhang, Y., Song, S., et al. Recent advances in the bioanalytical methods of polyethylene glycols and PEGylated pharmaceuticals. J. Sep. Sci. 43(9-10), 1978-1997 (2020).

3. Li, X.-Y., Li, T.-H., Guo, J.-S., et al. PEGylation of bovine serum albumin using click chemistry for the application as drug carriers. Biotechnol. Prog. 28(3), 856-861 (2012).

4. Ferrado, J.B., Perez, A.A., Menegon, M., et al. PEGylation of genistein-loaded bovine serum albumin nanoparticles and its effect on in vitro cell viability and genotoxicity properties. Colloids Surf. B Biointerfaces 222, 113082 (2023).