For immunochemical detection of RSK1 (phospho-Thr359/Ser363)
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RSK1 (Phospho-Thr359/Ser363) Rabbit Monoclonal Antibody (Clone RM233)

Item No. 32193

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • p90 Ribosomal S6 Kinase 1 (phospho-T359/S363)
Immunogen
Peptide corresponding to RSK1 (phospho-Thr359/Ser363)
Clone Designation
RM233
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) RSK1 (phospho-Thr359/Ser363)(-) RSK1 without phosphorylation at Thr359/Ser363
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µl

    $411

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    Product Description

    p90 ribosomal S6 kinase 1 (RSK1) is a member of the RSK family of serine/threonine kinases that mediates RAS/RAF/MEK/ERK/MAPK signaling.1 It contains N- and C-terminal kinase domains connected by a linker region, which contains various residues, including threonine 359 (Thr359) and serine 363 (Ser363), that are subject to phosphorylation and lead to its activation. RSK1 is ubiquitously expressed and localized to the cytoplasm, where it is in a complex with its activator ERK1/2 in quiescent cells.1,2 Upon stimulation of receptor tyrosine kinases (RTKs) by a variety of cytokines, neurotransmitters, or hormones, RSK1 accumulates at the plasma membrane and is phosphorylated by ERK1/2 and 3-phosphoinositide-dependent protein kinase 1 (PDK1), resulting in dissociation of the ERK1/2-RSK1 complex and activation of RSK1.3,1,2 RSK1 phosphorylates a variety of substrates that have roles in several cellular processes, including cell growth, survival, and proliferation.2 Tumor RSK1 levels are increased in patients with breast or prostate cancer.1 Cayman’s RSK1 (Phospho-Thr359/Ser363) Rabbit Monoclonal Antibody (Clone RM233) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Casalvieri, K.A., Matheson, C.J., Backos, D.S., et al. Selective targeting of RSK isoforms in cancer. Trends Cancer 3(4), 302-312 (2017).

    2. Romeo, Y., Zhang, X., and Roux, P.P. Regulation and function of the RSK family of protein kinases. Biochem. J. 441(2), 553-569 (2012).

    3. Anjum, R., and Blenis, J. The RSK family of kinases: Emerging roles in cellular signalling. Nat. Rev. Mol. Cell Biol. 9(10), 747-758 (2008).