Active • Host: Insect cells • AA: TAK1 (1-303) and TAB1 (437-504) • Tag: N-terminal GST • MW: 69 kDa
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TAK1-TAB1 Fusion (human, recombinant)

Item No. 33990

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • TAK1:
  • MAP3K7
  • TGF1a
  • TGFβ-activated Kinase 1
  • Mitogen-activated Protein Kinase Kinase Kinase 7
  • Transforming Growth Factor-β-activated Kinase 1
  • TGF-β-activated Kinase 1
  • TAB1:
  • MAP3K7IP1
  • TAK1-binding Protein 1
  • TGF-β-activated Kinase 1 and MAP3K7-binding Protein 1
  • Mitogen-activated Protein Kinase Kinase Kinase 7-interacting Protein 1
Purity
≥93% estimated by SDS-PAGE
Source
Active recombinant N-terminal GST-tagged human TAK1 fused to TAB1 via a DFGGGGG peptide linker expressed in insect cells
Amino Acids
1-303 (TAK1) and 437-504 (TAB1)
MW
69 kDa
Formulation (Request formulation change)
40 mM Tris-HCl, pH 8.0, with 110 mM sodium chloride, 2.2 mM potassium chloride, 3 mM DTT, and 20% glycerol
UniProt Accession №
O43318 (TAK1), Q15750 (TAB1)
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    10 µg

    $467

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    Product Description

    TGF-β-activated kinase 1 (TAK1) is a member of the serine/threonine MAP3K family that has roles in immunity and inflammation.1,2 It binds to TAK1-binding protein 1 (TAB1) at its N-terminal kinase domain and to TAB2 and TAB3 at its C-terminal domain.1 TAK1 is ubiquitously expressed and constitutively associated with its activating binding partner TAB1, whereas it only associates with TAB2 and TAB3 following stimulation by upstream factors such as IL-1β.3,4,5,2 It is involved in innate and adaptive immunity and the inflammatory response.1,2 A TAK1-TAB1 fusion protein induces the activation of JNK, p38 MAPK, and IĸB kinase (IKK) and the production of IL-6 in cancer cells.5 Tak1 knockdown induces splenomegaly and increases serum levels of several interleukin cytokines in mice.6 Hyperactivation of TAK1-TAB complexes is associated with autoimmune diseases and tumorigenesis.2 Cayman’s TAK1-TAB1 Fusion (human, recombinant) protein can be used for enzyme activity assays.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Dai, L., Thu, C.A., Liu, X.-Y., et al. TAK1, more than just innate immunity. IUBMB Life 64(10), 825-834 (2012).

    2. Xu, Y.-R., and Lei, C.-Q. TAK1-TABs complex: A central signalosome in inflammatory responses. Front. Immunol. 11, 608976 (2021).

    3. Kondo, M., Osada, H., Uchida, K., et al. Molecular cloning of human TAK1 and its mutational analysis in human lung cancer. Int. J. Cancer 75(4), 559-563 (1998).

    4. Kishimoto, K., Matsumoto, K., and Ninomiya-Tsuji, J. TAK1 mitogen-activated protein kinase kinase kinase is activated by autophosphorylation within its activation loop. The Journal of Biological Chemisty 275(10), 7359-7364 (2000).

    5. Sakurai, H., Nishi, A., Sato, N., et al. TAK1-TAB1 fusion protein: A novel constitutively active mitogen-activated protein kinase kinase kinase that stimulates AP-1 and NF-kappaB signaling pathways. Biochem. Biophys. Res. Commun. 297(5), 1277-1281 (2002).

    6. Vink, P.M., Smout, W.M., Driessen-Engels, L.J., et al. In vivo knockdown of TAK1 accelerates bone marrow proliferation/differentiation and induces systemic inflammation. PLoS One 8(3), e57348 (2013).