Isoscopoletin

Isoscopoletin is a coumarin that has been found in D. fragrans and has diverse biological activities and is an active metabolite of scoparone (Item No. 20046).^1,2,3,4,5 It is formed from scoparone by the cytochrome P450 (CYP) isoform Cyp2c29 in mouse liver microsomes.⁵ Isoscopoletin is cytotoxic to A549 lung, MCF-7 breast, and HepG2 liver cancer cells (IC₅₀s = 5.25, 8.58, and 4.76 µM, respectively).¹ It is active against Gram-positive and Gram-negative bacteria, including S. aureus and E. coli, respectively, in agar diffusion assays.² Isoscopoletin inhibits 15-lipoxygenase (15-LO; IC₅₀ = 15.1 µM) and reduces paw thickness in a rat model of carrageenan-induced paw edema when administered at a dose of 0.1 mg/kg.^3,4

WARNING This product is not for human or veterinary use.

1. Zhao, D.-D., Zhao, Q.-S., Liu, L., et al. Compounds from Dryopteris fragrans (L.) Schott with cytotoxic activity. Molecules 19(3), 3345-3355 (2014).

2. Nazir, S., Li, B., Tahir, K., et al. Antimicrobial activity of five constituents isolated from Ranunculus muricatus. J. Med. Plants Res. 7(47), 3438-3443 (2013).

3. Deng, S., Palu, A.K., West, B.J., et al. Lipoxygenase inhibitory constituents of the fruits of noni (Morinda citrifolia) collected in Tahiti. J. Nat. Prod. 70(5), 859-862 (2007).

4. Selim, Y.A., and Ouf, N.H. Anti-inflammatory new coumarin from the Ammi majus L. Org. Med. Chem. Lett. 2(1), 1 (2012).

5. Meyer, R.P., Hagemeyer, C.E., Knoth, R., et al. Oxidative hydrolysis of scoparone by cytochrome P450 CYP2C29 reveals a novel metabolite. Biochem. Biophys. Res. Commun. 285(1), 32-39 (2001).