Active • Host: E. coli • AA: 32-154 • MW: 14 kDa
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sPLA2 (human, recombinant type IIE)

Item No. 37955

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • GIIE sPLA2
  • Group IIE Secretory Phospholipase A2
  • Phosphatidylcholine 2-Acylhydrolase 2E
  • Phospholipase A2 Group IIE
  • sPLA2-IIE
Purity
≥80% estimated by SDS-PAGE
Source
Active recombinant human sPLA2 (Type IIE) expressed in E. coli
Amino Acids
32-154
MW
14 kDa
Formulation (Request formulation change)
25 mM Tris, pH 7.5, with 100 mM potassium chloride and 10 mM calcium chloride
UniProt Accession №
Q9NZK7
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    25 µg

    $336

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    Product Description

    Secretory phospholipase A2 (sPLA2) (Type IIE) is a calcium-dependent PLA2 superfamily member encoded by PLA2G2E in humans.1 It is composed of a highly conserved calcium-binding loop, a catalytic domain, and a Type II-specific disulfide-containing C-terminal extension but lacks the N-terminal signal peptide found in sPLA2 (Type IB) and sPLA2 (Type X). It also contains a second calcium-binding site that is essential for its catalytic activity.2,3 sPLA2 (Type IIE) is expressed in the brain, heart, lung, and placenta, as well as in hair follicles.4 It catalyzes the hydrolysis of phospholipids at the sn-2 position, preferentially releasing oleic acid (Item Nos. 90260 | 24659) from phosphatidylglycerol (PG) but also linoleic acid (Item Nos. 90150 | 90150.1 | 21909) or oleic acid from phosphatidylethanolamine (PE). It is involved in lipid metabolism, inflammatory processes, and regulation of lipolysis in adipocytes.2,4,5 sPLA2 (Type IIE) protein is upregulated in the kidney, small intestine, and lung following LPS administration in mice.4 Cayman’s sPLA2 (human, recombinant type IIE) protein can be used for enzyme activity assays.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Murakami, M., Taketomi, Y., Sato, H., et al. Secreted phospholipase A2 revisited. J. Biochem. 150(3), 233-255 (2011).

    2. Hou, S., Xu, T., Xu, J., et al. Structural basis for functional selectivity and ligand recognition revealed by crystal structures of human secreted phospholipase A2 group IIE. Sci. Rep. 7(1), 10815 (2017).

    3. Hou, S., Bai, J., Chen, C., et al. The atypical binding mechanism of second calcium on phospholipase A2 group IIE. Biochem. Biophys. Res. Commun. 557, 267-272 (2021).

    4. Suzuki, N., Ishizaki, J., Yokota, Y., et al. Structures, enzymatic properties, and expression of novel human and mouse secretory phospholipase A2s. The Journal of Biological Chemisty 275(8), 5785-5793 (2000).

    5. Zhi, H., Qu, L., Wu, F., et al. Group IIE secretory phospholipase A2 regulates lipolysis in adipocytes. Obesity (Silver Spring) 23(4), 760-768 (2015).