An ALC-0315-based LNP solution for expressing mCherry
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LipidLaunch™ ALC-0315 LNP (mCherry)

Item No. 40103

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Emission
610 nm
Excitation
587 nm
Formulation (Request formulation change)
TBS, pH 7.5, with 10% sucrose
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    5 µg

    $395

    Add
    Cayman Chemical
    Product Description

    LipidLaunch™ ALC-0315 LNP (mCherry) is a solution containing lipid nanoparticles (LNPs) composed of the ionizable cationic amino lipid ALC-0315 (Item No. 34337), cholesterol (Item No. 9003100), 1,2-distearoyl-sn-glycero-3-PC (Item No. 15100), and the lipid excipient ALC-0159 (Item No. 34336) at a molar ratio of 46.3:42.7:9.4:1.6 and encapsulating mRNA encoding the fluorescent protein mCherry. It is intended for proof-of-concept experiments to determine whether ALC-0315-based LNPs can effectively lead to the expression of a protein of interest in a target cell type, either in vitro or in vivo. mCherry displays excitation/emission maxima of 587/610 nm, respectively.

    Suggested in vitro use: Thaw LNPs on ice with occasional gentle swirling (do not vortex). Using a gentle pipetting technique, dilute 1:100-1:500 in complete cell culture media (with serum) and add to subconfluent adherent cells in a fluorescence imaging-compatible tissue culture plate. Expression of mCherry may be detectable as early as six hours after treatment. Optimal conditions are highly dependent on cell type.

    WARNING This product is not for human or veterinary use.