A lysosome-specific fluorescent reporter for the detection of hydroxyl radicals
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

HCy-Lyso

Item No. 41179

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
(E)-4-(4-(2-(1-ethyl-3,3-dimethylindolin-2-yl)vinyl)phenyl)morpholine
Molecular Formula
C24H30N2O
Formula Weight
362.5
Purity
≥98%
Emission
592 nm
Excitation
510 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
Chloroform: Sparingly soluble: 1-10 mg/ml
SMILES
CC1(C)C(/C=C/C2=CC=C(C=C2)N3CCOCC3)N(CC)C4=CC=CC=C14
InChi Code
InChI=1S/C24H30N2O/c1-4-26-22-8-6-5-7-21(22)24(2,3)23(26)14-11-19-9-12-20(13-10-19)25-15-17-27-18-16-25/h5-14,23H,4,15-18H2,1-3H3/b14-11+
InChi Key
VOKCJOWBCAYZKI-SDNWHVSQSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    1 mg

    $39

    Add

    5 mg

    $174

    Add

    10 mg

    $328

    Add
    Product Description

    HCy-Lyso is a lysosome-specific fluorescent reporter for the detection of hydroxyl radicals.1 It selectively fluoresces in low pH environments in the presence of hydroxy radicals over hypochlorite, superoxide, nitric oxide, or peroxynitrite radicals. HCy-Lyso (10 µM) displays lysosomal fluorescence in the presence of the ferroptosis inducers erastin (Item No. 17754) or (1S,3R)-RSL3 (Item No. 19288), an effect that can be blocked by the ferroptosis inhibitor ferrostatin-1 (Item No. 17729), in 4T1 cells. HCy-Lyso displays excitation/emission maxima of 510/592 nm, respectively, in low pH environments in the presence of hydroxy radicals.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Zhong, L., Fu, D., Xu, J., et al. Rational design of a lysosome-targeted fluorescent probe for monitoring the generation of hydroxyl radicals in ferroptosis pathways. RSC Adv. 14(18), 12864-12872 (2024).