For immunochemical detection of H2BK5Ac
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Histone H2BK5Ac Rabbit Monoclonal Antibody (Clone RM455)

Item No. 42790

Product Insert (PDF)
Technical Information
Synonyms
  • Acetylated Histone H2B Lysine 5
Immunogen
Peptide corresponding to H2BK5Ac
Clone Designation
RM455
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, multiplex-based assay, WB
Cross Reactivity
(+) H2BK5Ac(-) Unmodified H2BK5(-) H2B11Ac(-) H2BK12Ac(-) H2BK15Ac(-) H2BK16Ac(-) H2BK20Ac
Species Reactivity
(+) Human(+) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $501

    Add
    Cayman Chemical
    Explore Obesity & Metabolic Disease Resources

    Discover high-quality research tools to investigate GLP-1 mechanisms and next-generation metabolic targets.

    OBESITY RESEARCH SOLUTIONS
    Product Description

    Histone H2B is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains a histone fold domain with a C-terminal α-helix that facilitates nucleosome interactions and chromatin compaction, as well as an unstructured N-terminal tail that extends outside of the nucleosome core, both of which are subject to various post-translational modifications (PTMs), including ubiquitination, acetylation, methylation, and phosphorylation.1,2,3 Acetylation of histone H2B at lysine 5 (H2BK5Ac) promotes the expression of genes associated with maintenance of the epithelial phenotype in trophoblast stem cells and stem-like claudin-low breast cancer cells.4 Histone H2BK5Ac is enriched at cardiac and renal promotors in diabetic rats.5 Cayman’s Histone H2BK5Ac Rabbit Monoclonal Antibody (Clone RM455) can be used for ELISA, multiplex-based assay, and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    2. Wyrick, J.J., and Parra, M.A. The role of histone H2A and H2B post-translational modifications in transcription: A genomic perspective. Biochim. Biophys. Acta 1789(1), 37-44 (2009).

    3. Wang, C.-Y., Hua, C.-Y., Hsu, H.-E., et al. The C-terminus of histone H2B is involved in chromatin compaction specifically at telomeres, independently of its monoubiquitylation at lysine 123. PLoS One 6(7), e22209 (2011).

    4. Mobley, R.J., and Abell, A.N. Controlling epithelial to mesenchymal transition through acetylation of histone H2BK5. J. Nat. Sci. 3(9), e432 (2017).

    5. Malek, V., Sharma, N., and Gaikwad, A.B. Histone acetylation regulates natriuretic peptides and neprilysin gene expressions in diabetic cardiomyopathy and nephropathy. Curr. Mol. Pharmacol. 12(1), 61-71 (2019).