For immunochemical detection of His-tagged proteins
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His-Tag Rabbit Monoclonal Antibody (Clone RM146X)

Item No. 42809

Product Insert (PDF)
Technical Information
Synonyms
  • Deca-His Tag
  • Decahistidine Tag
  • Hexa-His Tag
  • 6x-His Tag
  • Hexahistidine Tag
  • Poly-His Tag
  • Polyhistidine Tag
Immunogen
6xHis-tag coupled to KLH
Clone Designation
RM146X
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, ICC, IP, WB
Cross Reactivity
(+) C-terminal His-tagged recombinant proteins(+) N-terminal His-tagged recombinant proteins(-) Very low non-specific reactivity with endogenous proteins in mammalian or bacterial cells compared with other His-Tag antibodies
Species Reactivity
(+) Species independent
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µg

    $438

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    Product Description

    His-Tag Rabbit Monoclonal Antibody is a probe for the immunochemical detection of histidine tags on recombinant proteins. Recombinant proteins are commonly labeled with affinity tags, such as 6-10 histidine residues (6x-10x His), to facilitate both their detection and purification.1 Poly-His tags are commonly utilized because of their small size, low potential to interfere in protein folding or activity, weak immunogenicity, and high affinity for transition metal ion matrices, such as Ni2+, used in immobilized metal-affinity chromatography (IMAC) for protein purification.1,2. Cayman’s His-Tag Rabbit Monoclonal Antibody (Clone RM146X) can be used for ELISA, immunocytochemistry (ICC), immunoprecipitation (IP), and Western blot (WB) applications. The antibody recognizes proteins containing His-tags fused to either the N- or C-terminus.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Terpe, K. Overview of tag protein fusions: From molecular and biochemical fundamentals to commercial systems. Appl. Microbiol. Biotechnol. 60(5), 523-533 (2003).

    2. Spriestersbach, A., Kubicek, J., Schäfer, F., et al. Purification of His-tagged proteins. Methods in Enzymology 559, 1-15 (2015).