For immunochemical detection of ERK2
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ERK2 Rabbit Monoclonal Antibody (Clone RM483)

Item No. 42840

Product Insert (PDF)
Technical Information
Synonyms
  • p42 MAPK
  • MAPK1
Immunogen
Peptide corresponding to the C-terminus of human ERK2
Clone Designation
RM483
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC and WB
Cross Reactivity
(+) ERK2
Species Reactivity
(+) Human(+) mouse(+) rat
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µL

    $411

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    Product Description

    ERK2 is a serine/threonine protein kinase and an essential component of the MAP kinase signaling cascade.1 It is composed of a glycine-rich loop, hinge region, and protein kinase domain containing a catalytic HRD motif, activation segment, and kinase insert domain. ERK2 is ubiquitously expressed with higher levels in the brain and hematopoietic cells.2,3 It localizes to the cytosol anchored to MEK1/2.1 Upon extracellular stimulation with mitogens, growth factors, or cytokines, the MAP kinase signaling cascade is initiated and terminates with phosphorylation of ERK2 in the activation segment's activation lip at Tyr204 or Tyr187 by MEK1/2.4,5,1,6 Upon activation, ERK2 phosphorylates and activates substrates in the cytosol or various subcellular compartments, including the nucleus, where they induce activation of transcription factors involved in cell proliferation, differentiation, and the stress response.7,8,4,5 Misregulation of the MAP kinase cascade drives the development of various cancers.4,5 Cayman's ERK2 Rabbit Monoclonal Antibody (Clone RM483) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Roskoski, R., Jr. ERK1/2 MAP kinases: Structure, function, and regulation. Pharm. Res. 66(2), 105-143 (2012).

    2. Buscá, R., Pouysségur, J., and Lenormand, P. ERK1 and ERK2 map kinases: Specific roles or functional redundancy? Front. Cell Dev. Biol. 4, 53 (2016).

    3. Vantaggiato, C., Formentini, I., Bondanza, A., et al. ERK1 and ERK2 mitogen-activated protein kinases affect Ras-dependent cell signaling differentially. J. Biol. 5(5), 14 (2006).

    4. Santarpia, L., Lippman, S.L., and El-Naggar, A.K. Targeting the mitogen-activated protein kinase RAS-RAF signaling pathway in cancer therapy. Expert. Opin. Ther. Targets 16(1), 103-119 (2012).

    5. Guo, Y.-J., Pan, W.-W., Liu, S.-B., et al. ERK/MAPK signalling pathway and tumorigenesis. Exp. Ther. Med. 19(3), 1997-2007 (2020).

    6. Buscá, R., Pouysségur, J., and Lenormand, P. ERK1 and ERK2. Encyclopedia of signaling molecules (2018).

    7. Lenormand, P., Brondello, J.-M., Brunet, A., et al. Growth factor-induced p42/p44 MAPK nuclear translocation and retention requires both MAPK activation and neosynthesis of nuclear anchoring proteins. J. Cell Biol. 142(3), 625-633 (1998).

    8. Brunet, A., Roux, D., Lenormand, P., et al. Nuclear translocation of p42/p44 mitogen-activated protein kinase is required for growth factor-induced gene expression and cell cycle entry. EMBO J. 18(3), 664-674 (1999).