Active • Host: Insect cells • AA: 1,060-1,620 • Tag: N-terminal GST • MW: ~90 kDa
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ALK/CD246 Intracellular Domain (L1196M mutant; human, recombinant)

Item No. 43700

Product Insert (PDF)
Technical Information
Synonyms
  • ALK Tyrosine Kinase Receptor
  • Anaplastic Lymphoma Kinase
  • CD246
  • Cluster of Differentiation 246
Purity
≥70% estimated by SDS-PAGE
Source
Active recombinant human N-terminal GST-tagged ALKL1196M intracellular domain expressed in insect cells
Amino Acids
1,060-1,620
MW
~90 kDa
Formulation (Request formulation change)
Sterile 50 mM Tris-HCl, pH 7.5, 50-300 mM sodium chloride, 10 mM glutathione, 0.1 mM EDTA, 0.25-1.0 mM DTT, 0-0.1 mM PMSF, and 10-25% glycerol
Host
Insect cells
UniProt Accession №
Q9UM73
Shipping & Storage Information
Shipping
in continental US; may vary elsewhere
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    10 µg

    $377

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    50 µg

    $1,317

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    500 µg

    $2,822

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    Product Description

    Anaplastic lymphoma kinase (ALK), also known as CD246, is a receptor tyrosine kinase and member of the insulin receptor superfamily.1 It is composed of an N-terminal extracellular domain that participates in cell-cell interactions, as well as transmembrane and juxtamembrane domains, and a C-terminal intracellular region that contains the protein kinase domain. Full-length ALK is expressed primarily in the embryonic nervous system, but the ALK kinase domain is expressed in a variety of cell types in the adult as a fusion protein.1,2 ALK is involved in cell proliferation and survival through downstream activation of the ERK1/2 and JAK/STAT signaling pathways, respectively.1 The fusion protein nucleophosmin-ALK (NPM-ALK) has a constitutively active ALK kinase domain and is ectopically expressed in anaplastic large-cell lymphoma (ALCL), a type of non-Hodgkin lymphoma.3 A variety of ALK-based fusion proteins are associated with ALCL, non-small cell lung cancer (NSCLC), inflammatory myofibroblastic tumor (IMT), diffuse large B cell lymphoma (DLBCL), and other cancers.1 Activating mutations in, and overexpression of, ALK have been associated with childhood neuroblastomas. Mutations in the gatekeeper residue Leu1196 to a methionine (L1196M) are associated with anticancer drug resistance.4,1 Cayman’s ALK/CD246 Intracellular Domain (L1196M mutant; human, recombinant) protein can be used for enzyme activity assays.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Roskoski, R., Jr. Anaplastic lymphoma kinase (ALK): Structure, oncogenic activation, and pharmacological inhibition. Pharmacol. Res. 68(1), 68-94 (2013).

    2. Vernersson, E., Khoo, N.K.S., Henriksson, M.L., et al. Characterization of the expression of the ALK receptor tyrosine kinase in mice. Gene Expr. Patterns 6(5), 448-461 (2006).

    3. Morris, S.W., Kirstein, M.N., Valentine, M.B., et al. Fusion of a kinase gene, ALK, to a nucleolar protein gene, NPM, in non-Hodgkin’s lymphoma. Science 263(5151), 1281-1284 (1994).

    4. Mah, S., Park, J.H., Jung, H.-Y., et al. Identification of 4-phenoxyquinoline based inhibitors for L1196M mutant of anaplastic lymphoma kinase by structure-based design. J. Med. Chem. 60(22), 9205-9221 (2017).