Active • Host: Yeast • AA: 82-190 • Tag: N-terminal His • MW: 14.3 kDa
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PDGF-B (human, recombinant)

Item No. 44191

Product Insert (PDF)
Technical Information
Synonyms
  • Becaplermin
  • IBGC5
  • PDGF-2
  • PDGFB
  • Platelet-Derived Growth Factor B Chain
  • Platelet-Derived Growth Factor β Polypeptide
  • Proto-oncogene c-Sis
  • SIS
  • SSV
Purity
≥90% estimated by SDS-PAGE
Endotoxin Testing
<1.0 EU/μg, determined by the LAL endotoxin assay
Source
Active recombinant human N-terminal His-tagged PDGF-B expressed in yeast
Amino Acids
82-190
MW
14.3 kDa
Formulation (Request formulation change)
Lyophilized from sterile 30% acetonitrile and 1/1,000 trifluoroacetic acid
UniProt Accession №
P01127
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    20 µg

    $158

    Add

    50 µg

    $296

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    1 mg

    $2,370

    Add
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    Product Description

    PDGF-B is a secreted growth factor and member of the PDGF family.1 It is composed of a signal peptide, a pro-peptide sequence, a growth factor domain containing a cystine knot, and a C-terminal tail. PDGF-B is expressed at high levels in the heart, placenta, and fetal kidney and in the brain in the substantia nigra.2 It exists as either a homodimer, PDGF-BB, which can activate PDGFRα or PDGFRβ, or a heterodimer with PDGF-A, PDGF-AB, which can only activate PDGFRα, and induces signaling through multiple pathways, including ERK/MAPK, PI3K/Akt/mTOR, PLC/PKC, and JAK/STAT.1,3,4 PDGF-B is a potent mitogen for cells of mesenchymal origin and is necessary for vascular development and vascular smooth muscle cell proliferation in the CNS, lungs, heart, placenta, and skin.4,5 A fusion between the genes encoding PDGF-B and collagen 1A1, PDGFB and COL1A1, leads to overproduction of PDGF-B and is associated with various benign and malignant tumors, including dermatofibrosarcoma protuberans.4 Formulations containing PDGF-B have been used in the treatment of diabetic neuropathic foot ulcers. Cayman's PDGF-B (human, recombinant) protein can be used for binding assays. This protein consists of 124 amino acids and has a calculated molecular weight of 14.3 kDa. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is 20 kDa due to glycosylation.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Chen, P.-H., Chen, X., and He, X. Platelet-derived growth factors and their receptors: Structural and functional perspectives. Biochim. Biophys. Acta 1834(10), 2176-2186 (2013).

    2. LaRochelle, W.J., Jeffers, M., McDonald, W.F., et al. PDGF-D, a new protease-activated growth factor. Nat. Cell Biol. 3(5), 517-521 (2001).

    3. Heldin, C.-H., and Lennartsson, J. Structural and functional properties of platelet-derived growth factor and stem cell factor receptors. Cold Spring Harb. Perspect. Biol. 5(8), a009100 (2013).

    4. Roskoski, R., Jr. The role of small molecule platelet-derived growth factor receptor (PDGFR) inhibitors in the treatment of neoplastic disorders. Pharmacol. Res. 129, 65-83 (2018).

    5. Gaengel, K., Genové, G., Armulik, A., et al. Endothelial-mural cell signaling in vascular development and angiogenesis. Arterioscler. Thromb. Vasc. Biol. 29(5), 630-638 (2009).