A triphenylmethane dye
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Methyl Green

Item No. 44933

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
4-[[4-(dimethylamino)phenyl][4-(dimethyliminio)-2,5-cyclohexadien-1-ylidene]methyl]-N,N,N-trimethyl-benzenaminium, dichloride
CAS Number
82-94-0
Synonyms
  • C.I. 42585
  • Double Green SF
  • Light Green
Molecular Formula
C26H33N3 • 2Cl
Formula Weight
458.5
Emission
682 nm, in the presence of DNA
Excitation
633 nm, in the presence of DNA
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: Sparingly soluble: 1-10 mg/mlPBS (pH 7.2): Soluble: ≥ 10 mg/ml
SMILES
C/[N+](C)=C(C=C/1)/C=CC1=C(C2=CC=C([N+](C)(C)C)C=C2)/C3=CC=C(N(C)C)C=C3.[Cl-].[Cl-]
InChi Code
InChI=1S/C26H33N3.2ClH/c1-27(2)23-14-8-20(9-15-23)26(21-10-16-24(17-11-21)28(3)4)22-12-18-25(19-13-22)29(5,6)7;;/h8-19H,1-7H3;2*1H/q+2;;/p-2
InChi Key
DWCZIOOZPIDHAB-UHFFFAOYSA-L
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Certificates of Analysis & Batch Specific Data

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    1 g

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    10 g

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    Cayman Chemical
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    Product Description

    Methyl green is a triphenylmethane dye.1,2 It displays an excitation maximum of 633 nm in the presence or absence of DNA and an emission maximum of 682 nm only in the presence of DNA.2 Methyl green has commonly been used to distinguish DNA from RNA when used in combination with pyronin Y (Item No. 14488) and as a histological and cell viability stain.1,2 It has been found in wastewater.3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Lyon, H., Schulte, E., de Leenheer, A., et al. Dye standards, Part II. 2: Methyl green (CI 42585) and ethyl green (CI 42590). Histochem J. 24(4), 224-227 (1992).

    2. Murgai, P., Sharma, P., Sachdeva, M.U.S., et al. A flow cytometric cell-cycle assay using methyl green. Anal. Biochem. 601, 113782 (2020).

    3. Ikram, M., Mutahir, S., Humayun, M., et al. Facile synthesis of ZIF-67 for the adsorption of methyl green from wastewater: Integrating molecular models and experimental evidence to comprehend the removal mechanism. Molecules 27(23), 8385 (2022).