Firefly Luciferase circRNA

Firefly luciferase (FLuc) circular RNA (circRNA) encodes firefly luciferase, a protein originally isolated from the firefly P. pyralis, that catalyzes the ATP-dependent oxidation of the molecule luciferin (D-luciferin; Item Nos. 25836 | 14682 | 14681) resulting in chemiluminescence at a wavelength of approximately 560 nm.¹ Exogenous circRNAs have increased stability and can produce sustained protein expression compared to mRNA.² Firefly luciferase circRNA has been used as a reporter for the delivery of circRNA via lipid nanoparticles (LNPs).³

WARNING This product is not for human or veterinary use.

1. Baldwin, T.O. Firefly luciferase: The structure is known, but the mystery remains. Structure 4(3), 223-228 (1996).

2. Wesselhoeft, R.A., Kowalski, P.S., and Anderson, D.G. Engineering circular RNA for potent and stable translation in eukaryotic cells. Nat. Commun. 9(1), 2629 (2018).

3. Alshehry, Y., Liu, X., Zhang, Y., et al. Investigation of the impact of lipid nanoparticle compositions on the delivery and T cell response of circRNA vaccine. J. Control Release 381, 113617 (2025).