L-Leucine-d₃

L-Leucine-d₃ is intended for use as an internal standard for the quantification of L-leucine (Item No. 34342) by GC- or LC-MS. L-Leucine is an essential amino acid.¹ It increases basal insulin secretion and decreases glucose-induced insulin release in INS-1E rat insulinoma cells when used at concentrations of 1, 5, and 10 mM.² L-Leucine (1, 5, and 10 mM) decreases triglyceride levels and increases cholesterol accumulation in INS-1E cells. It stimulates skeletal muscle protein synthesis in exercised rats, as well as in food-deprived rats in an mTOR-dependent manner.^3,4 Formulations containing L-leucine have been used as dietary supplements.

WARNING This product is not for human or veterinary use.

1. Duan, Y., Li, F., Li, Y., et al. The role of leucine and its metabolites in protein and energy metabolism. Amino Acids 48(1), 41-51 (2016).

2. Liu, Z., Jeppesen, P.B., Gregersen, S., et al. Chronic exposure to leucine in vitro induces β-cell dysfunction in INS-1E cells and mouse islets. J. Endocrinol. 215(1), 79-88 (2012).

3. Anthony, J.C., Yoshizawa, F., Anthony, T.G., et al. Leucine stimulates translation initiation in skeletal muscle of postabsorptive rats via a rapamycin-sensitive pathway. J. Nutr. 130(10), 2413-2419 (2000).

4. Anthony, J.C., Anthony, T.G., and Layman, D.K. Leucine supplementation enhances skeletal muscle recovery in rats following exercise. J. Nutr. 129(6), 1102-1106 (1999).