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Expression System Bacterial Insect Mammalian

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Protein Production

Backed by years of experience and expertise, Cayman offers comprehensive recombinant protein production services that include multiple expression platforms, purification methods, and characterization services to meet your project’s specific needs. This complements a catalog of hundreds of recombinant proteins and antibodies. As a Cayman client, you remain in control of your project while we provide a flexible, phase-based approach to help achieve your project goals.

Cayman’s scientists will ensure that your protein is engineered to exact specifications and produced using the optimal bacterial, insect, or mammalian expression system. Choose from a selection of expression-ready constructs or have our team develop one specific to your project.

Phase 1Construct Design, Gene Synthesis, and Cloning(3-4 weeks)

In silico clone design
Sequence confirmation
Expression plasmid production

Phase 2Expression Optimization(2 weeks)

Expression system selection and optimization:
- Bacterial
- Insect
- Mammalian

See table below for more information about expression systems

Phase 3Pilot Development(time varies)

1 L biomass production
Purification method development:
- Immobilized metal-affinity chromatography (IMAC) and affinity chromatography
- Ion exchange chromatography (IEX)
- Hydrophobic interaction chromatography (HIC)
- Size-exclusion chromatography (SEC)
- Other

Phase 4Protein Production(2 weeks per round)

Large-scale cell growth
Upscaled purification for manufacture of high-yield, high-purity targets
Up to gram quantities for select proteins
Formulation into final desired buffer

Phase 5Protein Characterization(time varies)

Purity (SDS-PAGE)
Identity (Western blot (WB), mass spectrometry, isoelectric focusing (IEF) gel analysis)
Quantity (BCA assay, Bradford assay, A280)
Size homogeneity (size-exclusion chromatography)
Functionality (activity assays, surface plasmon resonance (SPR))
Stability (thermal shift analysis)
Formulation optimization using thermal shift assay (TSA)
Endotoxin determination and depletion
Freeze/thaw analysis
Dynamic light scattering (DLS)

Expression Systems

Our expert scientists can help you determine the best expression system for your custom recombinant protein project.

	Expression Systems
	Bacterial	Insect	Mammalian
Advantages	Inexpensive, fast, and easy	More effective for difficult targets	More effective for difficult targets
Limitations	Solubility/inclusion body issues Endotoxins No PTMs	Baculovirus expression vector production and amplification is time-consuming Requires specific and rigorous culture conditions	Requires specific and rigorous culture conditions
Common Applications	Scalable expression of bacterial and moderately complicated targets	Expression of protein targets requiring post-translational modification Transmembrane targets Secreted proteins	Expression of protein targets requiring post-translational modification Recombinant antibodies Secreted proteins
Post-Translational Modifications (PTMs)	No	Yes	Yes
Protein Yield	~1-40 mg/L	~1-25 mg/L	~10-50 mg/L (stable) ~300-700 mg/L (transient)
Cost	$	$$	$$$

Pricing

Pricing for our recombinant protein production services varies based on each project’s unique specifications, including the expression system, purification method, and characterization services used. You can request more information, including project-specific pricing, by submitting the “Contact Us” form.

Additional Services

Native Protein Isolation

Purification from microbial, plant, and animal sources.

PTM Mapping

Sensitive, site-specific determination of PTMs using LC-MS/MS.

Protein Labeling

In vitro conjugation of proteins to a variety of labels and probes.

Modified Recombinant Proteins

Enzyme-directed citrullination, carbamylation, phosphorylation, and additional modifications under carefully optimized conditions.

Related Services

Antibody Production

Customized antigen synthesis, affinity purification, and quality guarantees go into the design of each polyclonal antibody, monoclonal antibody, or antiserum produced from immunized rabbits or other animals.

Structure-Based Drug Design

Robust biophysical characterization, crystallization and X-ray structure determination, SPR binding analysis, in silico docking, and homology modeling.

Biophysical Characterization

We offer a variety of biophysical methods, including surface plasmon resonance (SPR), thermal shift assay (TSA), and dynamic light scattering (DLS), to help you characterize small molecules and proteins, understand molecular interactions and binding mechanisms, and optimize drug-target interactions.

Custom Assay Development

Have quantitative assays built on a variety of analysis and detection platforms to suit your specific needs.

Proteomic Profiling

Mass spectrometry-based quantitative proteomics.

See how our recombinant protein modification and characterization services have been used

View the poster

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