A ROCK inhibitor
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Y-27632 (hydrochloride)

Item No. 10005583

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
trans-4-[(1R)-1-aminoethyl]-N-4-pyridinyl-cyclohexanecarboxamide, dihydrochloride
CAS Number
129830-38-2
Molecular Formula
C14H21N3O • 2HCl
Formula Weight
320.3
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 1 mg/mlDMSO: 30 mg/mlEthanol: 1 mg/mlPBS (pH 7.2): 100 mg/mlWater: 30 mg/ml
λmax
270 nm
SMILES
O=C([C@H]1CC[C@@]([C@H](N)C)([H])CC1)NC2=CC=NC=C2.Cl.Cl
InChi Code
InChI=1S/C14H21N3O.2ClH/c1-10(15)11-2-4-12(5-3-11)14(18)17-13-6-8-16-9-7-13;;/h6-12H,2-5,15H2,1H3,(H,16,17,18);2*1H/t10-,11-,12-;;/m1../s1
InChi Key
IDDDVXIUIXWAGJ-LJDSMOQUSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

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    5 mg

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    10 mg

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    50 mg

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    Product Description

    Y-27632 is a potent, ATP-competitive inhibitor of ROCKs including p160ROCK (Ki = 140 nM) and ROCK2 (IC50 = 800 nM). It also inhibits PRK2 with an IC50 value of 600 nM.1,2 One-hour treatment with 10 µM Y-27632 blocks apoptosis of dissociated cultured human embryonic stem cells, increasing cloning efficiency by 25% and sustaining survival up to 30 passages.3 This product is also available as part of Cayman's Astrocyte Reprogramming Reagent Kit (DFICBY) (Item No. 502887).

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Davies, S.P., Reddy, H., Caivano, M., et al. Specificity and mechanism of action of some commonly used protein kinase inhibitors. Biochem. J. 351(1), 95-105 (2000).

    2. Uehata, M., Ishizaki, T., Satoh, H., et al. Calcium sensitization of smooth muscle mediated by a Rho-assocciated protein kinase in hypertension. Nature 389, 990-994 (1997).

    3. Watanabe, K., Ueno, M., Kamiya, D., et al. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat. Biotechnol. 25(6), 681-686 (2007).

    Product Citations

    Greda, A.K., Gomes, J.P., Schmidt-Krueger, V., et al. Interaction of sortilin with apolipoprotein E3 enables neurons to use long-chain fatty acids as alternative metabolic fuel. Nat. Metab. 7(11), 2346-2365 (2025).