LDL Receptor Polyclonal Antibody

LDL receptor (LDLR) is a cell surface glycoprotein that scavenges LDL from the blood and regulates plasma LDL levels.¹ It is composed of an N-terminal signal sequence, a ligand-binding domain, an EGF precursor homology domain, an O-linked glycosylation domain, a transmembrane region, and a C-terminal cytoplasmic tail. LDLR is primarily expressed in the liver but is also found in the adrenal cortex.² It mediates the endocytosis of LDL by binding to apolipoprotein E (ApoE) or ApoB on the LDL surface, thereby supplying cholesterol to cells.¹ Protein levels of LDLR are decreased in HepG2 cells expressing proprotein convertase subtilisin kexin 9 (PCSK9).³ Knockout of Ldlr increases plasma levels of cholesterol and triglycerides and induces the formation of atherosclerotic lesions in mice.⁴ Mutations in LDLR are associated with familial hypercholesterolemia.⁵ Cayman's LDL Receptor Polyclonal Antibody can be used for immunocytochemistry (ICC), immunohistochemistry (IHC), and Western blot (WB) applications.

WARNING This product is not for human or veterinary use.

1. Goldstein, J.L., Brown, M.S., Anderson, R.G.W., et al. Receptor-mediated endocytosis: Concepts emerging from the LDL receptor system. Annu. Rev. Cell Biol. 1, 1-39 (1985).

2. Rudling, M.J., Reihnér, E., Einarsson, K., et al. Low density lipoprotein receptor-binding activity in human tissues: Quantitative importance of hepatic receptors and evidence for regulation of their expression in vivo. Proc. Natl. Acad. Sci. USA 87(9), 3469-3473 (1990).

3. Benjannet, S., Rhainds, D., Essalmani, R., et al. NARC-1/PCSK9 and its natural mutants. Zymogen cleavage and effects on the low density lipoprotein (LDL) receptor and LDL cholesterol. The Journal of Biological Chemisty 279(47), 48865-48875 (2004).

4. Praticò, D., Tillmann, C., Zhang, Z.B., et al. Acceleration of atherogenesis by COX-1-dependent prostanoid formation in low density lipoprotein receptor knockout mice. Proc. Natl. Acad. Sci. USA 98(6), 3358-3363 (2001).

5. Austin, M.A., Hutter, C.M., Zimmern, R.L., et al. Genetic causes of monogenic heterozgous familial hypercholesterolemia: A HuGE prevalence review. Am. J. Epidemiol. 160(5), 407-420 (2004).

Pommier, A.J.C., Dufour, J., Alves, G., et al. Liver X receptors protect from development of prostatic intra-epithelial neoplasia in mice. PLoS Genet. 9(5), e1003483 (2013).

Hong, C., Duit, S., Jalonen, P., et al. The E₃ ubiquitin ligase IDOL induces the degradation of the low density lipoprotein receptor family members VLDLR and ApoER2. The Journal of Biological Chemisty 285(26), 19720-19726 (2010).