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N-desethyl Isotonitazene (hydrochloride)

Item No. 30216

Restricted Product (Schedule I)
Product Insert (PDF)GC-MS (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-ethyl-2-[[4-(1-methylethoxy)phenyl]methyl]-5-nitro-1H-benzimidazole-1-ethanamine, monohydrochloride
Molecular Formula
C21H26N4O3 • HCl
Formula Weight
418.9
Purity
≥98%
Formulation
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 30 mg/mlEthanol: 30 mg/mlPBS (pH 7.2): 5 mg/ml
λmax
240, 301 nm
SMILES
CCNCCN1C(CC2=CC=C(OC(C)C)C=C2)=NC3=CC([N+]([O-])=O)=CC=C31.Cl
InChi Code
InChI=1S/C21H26N4O3.ClH/c1-4-22-11-12-24-20-10-7-17(25(26)27)14-19(20)23-21(24)13-16-5-8-18(9-6-16)28-15(2)3;/h5-10,14-15,22H,4,11-13H2,1-3H3;1H
InChi Key
YGIGEJHYUUWLMA-UHFFFAOYSA-N
Regulatory Information
DEA Schedule
I
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    Product Description

    N-desethyl Isotonitazene (hydrochloride) (Item No. 30216) is an analytical reference standard categorized as an opioid.1,2 N-desethyl Isotonitazene is a metabolite of isotonitazene (Item Nos. 30880 | 27255). N-desethyl Isotonitazene is regulated as a Schedule I compound in the United States. This product is intended for research and forensic applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Krotulski, A.J., Papsun, D.M., Kacinko, S.L., et al. Isotonitazene quantitation and metabolite discovery in authentic forensic casework. J. Anal. Toxicol. 44(6), 521-530 (2020).

    2. Vandeputte, M.M., Van Uytfanghe, K., Layle, N.K., et al. Synthesis, chemical characterization, and µ-opioid receptor activity assessment of the emerging group of "nitazene" 2-benzylbenzimidazole synthetic opioids. ACS Chem. Neurosci. 12(7), 1241-1251 (2021).

    Product Citations

    Scully, L., Castle, J.W., Di Rago, M., et al. Detection of nitazenes (2-benzylbenzimidazoles)—novel synthetic opioids in coronial casework in Victoria, Australia. Metabolites 16(6), 358 (2026).

    Hardwick, E.K., and Davidson, J.T. Structural characterization of nitazene analogs using electrospray ionization-tandem mass spectrometry (ESI-MS/MS). Drug Test Anal. (2025).

    Kozell, L.B., Eshelman, A., J., Wolfrum, K.M., et al. Pharmacologic characterization of substituted nitazenes at μ, κ, and Δ opioid receptors suggests high potential for toxicity. J. Pharmacol. Exp. Ther. 389(2), 219-228 (2024).

    Taoussi, O., Berardinelli, D., Zaami, S., et al. Human metabolism of four synthetic benzimidazole opioids: Isotonitazene, metonitazene, etodesnitazene, and metodesnitazene. Arch. Toxicol. (2024).

    Tsai, M.-H.M., Chen, L., Baumann, M.H., et al. In Vitro Functional Profiling of Fentanyl and Nitazene Analogs at the μ-Opioid Receptor Reveals High Efficacy for Gi Protein Signaling. ACS Chem. Neurosci. 15(4), 854-867 (2024).

    Monti, M.C., De Vrieze, L.M., Vandeputte, M.M., et al. Detection of N-desethyl etonitazene in a drug checking sample: Chemical analysis and pharmacological characterization of a recent member of the 2-benzylbenzimidazole "nitazene" class. J. Pharm. Biomed. Anal. 251:116453, (2024).

    Glatfelter, G.C., Vandeputte, M.M., Chen, L., et al. Alkoxy chain length governs the potency of 2-benzylbenzimidazole “nitazene” opioids associated with human overdose. Psychopharmacology (Berl.) (2023).

    Malcolm, N.J., Palkovic, B., Sprague, D.J., et al. Mu-opioid receptor selective superagonists produce prolonged respiratory depression. iScience 26(7), 107121 (2023).

    Vandeputte, M.M., Van Uytfanghe, K., Layle, N.K., et al. Synthesis, chemical characterization, and µ-opioid receptor activity assessment of the emerging group of "nitazene" 2-benzylbenzimidazole synthetic opioids. ACS Chem. Neurosci. 12(7), 1241-1251 (2021).