Cy5 Firefly Luciferase mRNA (Cap-1; mo⁵U)

Cy5 Firefly Luciferase mRNA (Cap-1; mo⁵U) encodes luciferase, a protein originally isolated from the firefly P. pyralis, that catalyzes the ATP-dependent oxidation of the molecule luciferin resulting in chemiluminescence at a wavelength of approximately 560 nm.¹ It is capped using a co-transcriptional capping method, resulting in the naturally occurring Cap 1 structure with high capping efficiency. Cy5 Firefly Luciferase mRNA is also polyadenylated and modified with 5-methyoxy-UTP (mo⁵U) to reduce the host cell immune response, increase mRNA stability, and enhance translation initiation. Cy5 (Item No. 30752) is a cyanine-containing fluorochrome with excitation/emission maxima of 650/670 nm, respectively.² Encapsulation of Cy5 Firefly Luciferase mRNA (Cap-1; mo⁵U) in lipid nanoparticles (LNPs) can be used for mRNA delivery and expression of luciferase protein in vitro.³

WARNING This product is not for human or veterinary use.

1. Baldwin, T.O. Firefly luciferase: The structure is known, but the mystery remains. Structure 4(3), 223-228 (1996).

2. Suzuki, T., Matsuzaki, T., Hagiwara, H., et al. Recent advances in fluorescent labeling techniques for fluorescence microscopy. Acta Histochem. Cytochem. 40(5), 131-137 (2007).

3. Li, J., Wu, Y., Xiang, J., et al. Fluoroalkane modified cationic polymers for personalized mRNA cancer vaccines. Chem. Eng. J. 456, 140930 (2023).