A BSA complex with palmitic acid-13C16
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BSA-13C16-Palmitate Saturated Fatty Acid Complex (5 mM)

Item No. 43209

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Bovine Serum Albumin-13C16-PA
  • Bovine Serum Albumin-13C16-Palmitate
  • BSA-13C16-PA
  • BSA-Hexadecanoic Acid-13C16
Formulation (Request formulation change)
5 mM 13C16-Palmitate:0.8 mM BSA (6:1 13C-palmitate:BSA) in 150 mM sodium chloride, pH 7.4
Origin
Animal/Bovine
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    5 ml

    $229

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    Product Description

    BSA-13C16-Palmitate Saturated Fatty Acid Complex (5 mM) is composed of palmitic acid-13C16 (Item No. 45789) and fatty acid-free bovine serum albumin (BSA) at an approximately 6:1 molar ratio of palmitate-13C16:BSA. Cayman’s BSA-Palmitate-13C16 Saturated Fatty Acid Complex (5 mM) can be used for mass spectrometry-based metabolic tracing, including in animal models of diabetes to assess palmitic acid incorporation into acylcarnitines and citric acid cycle metabolites.1,2 It can also be used for efficient fatty acid delivery to cells in culture for the purpose of monitoring fatty acid uptake, lipid metabolism, including lipid synthesis and fatty acid oxidation, and inflammatory signaling pathways.3,4,5 Cayman’s BSA-Palmitate-13C16 Saturated Fatty Acid Complex (5 mM) is suitable for use in short-term cell culture applications (acute treatment to 18 hours); however, for long-term applications (25+ hours) the product should be filter-sterilized using a 0.2 µm filter and sterile receptacle, which will not affect its performance. For best results, it is recommended that this product be used in conjunction with Cayman’s BSA Control for BSA-Fatty Acid Complexes (5 mM) (Item No. 29556), prepared with fatty acid-free BSA.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Li, J., and Cheng, J.-X. Direct visualization of de novo lipogenesis in single living cells. Sci. Rep. 4, 3807 (2014).

    2. Sas, K.M., Kayampilly, P., Byun, J., et al. Tissue-specific metabolic reprogramming drives nutrient flux in diabetic complications. JCI Insight 1(15), e86976 (2016).

    3. Alsabeeh, N., Chausse, B., Kakimoto, P.A., et al. Cell culture models of fatty acid overload: Problems and solutions. Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1863(2), 143-151 (2018).

    4. Kim, K.-S., Ko, Y.G., Yang, W.S., et al. A parallel reaction monitoring-mass spectrometric method for studying lipid biosynthesis in vitro using 13C16-palmitate as an isotope tracer. Anal. Chim. Acta 1354, 344003 (2025).

    5. Wang, D., Green, M.F., McDonnell, E., et al. Oxygen flux analysis to understand the biological function of sirtuins. Methods Mol. Biol. 1077, 241-258 (2013).