A BSA complex with palmitic acid-d9
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BSA-Palmitate-d9 Saturated Fatty Acid Complex (1 mM)

Item No. 43988

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Bovine Serum Albumin-PA-d9
  • Bovine Serum Albumin-Palmitate-d9
  • BSA-Hexadecanoic Acid-d9
  • BSA-PA-d9
Formulation (Request formulation change)
1 mM Palmitate-d9:0.8 mM BSA (6:1 palmitate-d9:BSA) in 150 mM sodium chloride, pH 7.4
Origin
Animal/Bovine
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    5 ml

    $229

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    Product Description

    BSA-Palmitate-d9 Saturated Fatty Acid Complex (1 mM) is composed of palmitic acid-d9 (Item No. 37061) and fatty acid-free bovine serum albumin (BSA) at an approximately 6:1 molar ratio of palmitate-d9:BSA. It is intended for the quantification of palmitate-d9 uptake and incorporation by GC- or LC-MS. Cayman’s BSA-Palmitate-d9 Saturated Fatty Acid Complex (1 mM) can be used for efficient fatty acid delivery to cells in culture for the purpose of monitoring lipid metabolism, including fatty acid oxidation, and inflammatory signaling pathways.1,2 It can also be used in fatty acid uptake assays and for metabolic tracing, including in the assessment of palmitic acid incorporation into phospholipids in vitro via imaging mass spectrometry.3,4 Cayman’s BSA-Palmitate-d9 Saturated Fatty Acid Complex (1 mM) is suitable for use in short-term cell culture applications (acute treatment to 18 hours); however, for long-term applications (25+ hours) the product should be filter-sterilized using a 0.2 µm filter and sterile receptacle, which will not affect its performance. For best results, it is recommended that this product be used in conjunction with Cayman’s BSA Control for BSA-Fatty Acid Complexes (1 mM) (Item No. 34932), prepared with fatty acid-free BSA.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Alsabeeh, N., Chausse, B., Kakimoto, P.A., et al. Cell culture models of fatty acid overload: Problems and solutions. Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1863(2), 143-151 (2018).

    2. Wang, D., Green, M.F., McDonnell, E., et al. Oxygen flux analysis to understand the biological function of sirtuins. Methods Mol. Biol. 1077, 241-258 (2013).

    3. Li, J., and Cheng, J.-X. Direct visualization of de novo lipogenesis in single living cells. Sci. Rep. 4, 3807 (2014).

    4. Sugiyama, E., Yao, I., and Setou, M. Visualization of local phosphatidylcholine synthesis within hippocampal neurons using a compartmentalized culture system and imaging mass spectrometry. Biochem. Biophys. Res. Commun. 495(1), 1048-1054 (2018).