EGFP circRNA

EGFP circular RNA (circRNA) encodes enhanced GFP (EGFP), a variant of A. victoria GFP containing chromophore mutations that increase the sensitivity of the protein and displays excitation/emission maxima of 489/511 nm, respectively.¹ Exogenous circRNAs have increased stability and can produce sustained protein expression compared to mRNA.² EGFP circRNA has been used as a control for the delivery of VEGF-A mRNA in human umbilical vein endothelial cells (HUVECs) via lipid nanoparticles (LNPs).³

WARNING This product is not for human or veterinary use.

1. Zhang, G., Gurtu, V., and Kain, S.R. An enhanced green fluorescent protein allows sensitive detection of gene transfer in mammalian cells. Biochem. Biophys. Res. Commun. 227(3), 707-711 (1996).

2. Wesselhoeft, R.A., Kowalski, P.S., and Anderson, D.G. Engineering circular RNA for potent and stable translation in eukaryotic cells. Nat. Commun. 9(1), 2629 (2018).

3. Liu, J., Zhang, Y., Liu, C., et al. A single dose of VEGF-A circular RNA sustains in situ long-term expression of protein to accelerate diabetic wound healing. J. Control Release 373, 319-335 (2024).