An mRNA for the expression of Mito-EGFP
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Product Categories

Product Type

Lipid Nanoparticles (LNPs)
Cargos

Research Area

Cell Biology
Mitochondrial Biology

Application

Fluorescence

Mito-EGFP Probe mRNA (Cap-1; m1Ψ)

Item No. 43683

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Mitochondria-EGFP mRNA (Cap-1; N1-Methylpseudouridine)
Emission
511 nm
Excitation
489 nm
Formulation
1 mM Sodium citrate, pH 6.4
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $303

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    1 mg

    $1,513

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    Cayman Chemical
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    Product Description

    Mito-EGFP Probe mRNA (Cap-1; m1Ψ) encodes a fusion protein composed of a mitochondrial targeting sequence (Mito) and enhanced GFP (EGFP), a variant of A. victoria GFP that contains chromophore mutations that increase the sensitivity of the protein and displays excitation and emission maxima of 489 and 511 nm, respectively.1 It is capped using a co-transcriptional capping method, resulting in the naturally occurring Cap-1 structure with high capping efficiency. Mito-EGFP probe mRNA is also polyadenylated and modified with N1-methylpseudouridine (m1Ψ) to reduce the host cell immune response and enhance mRNA stability. It can be used in the study of mitochondrial structure and function in live cells. Encapsulation of Mito-EGFP probe mRNA (Cap-1; m1Ψ) in lipid nanoparticles (LNPs) can be used for mRNA delivery and expression of Mito-EGFP.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Zhang, G., Gurtu, V., and Kain, S.R. An enhanced green fluorescent protein allows sensitive detection of gene transfer in mammalian cells. Biochem. Biophys. Res. Commun. 227(3), 707-711 (1996).