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EGFP mRNA (Cap-1; m1Ψ) encodes enhanced GFP (EGFP), a variant of A. Victoria GFP containing chromophore mutations that increase the sensitivity of the protein and displays excitation/emission maxima of 489/511 nm, respectively.1 EGFP mRNA (Cap-1; m1Ψ) is polyadenylated and modified with N1-methylpseudouridine (m1Ψ) to reduce the host cell immune response and enhance mRNA stability. EGFP mRNA (Cap-1; m1Ψ) can be used for mRNA delivery, cell viability assays, and in vivo imaging.
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1. An enhanced green fluorescent protein allows sensitive detection of gene transfer in mammalian cells. Biochem. Biophys. Res. Commun. 227(3), 707-711 (1996).