New publication in Nature Chemical Biology using Cayman's Cyclic di-AMP ELISA Kit

The cyclic dinucleotide (CDN) cyclic di-AMP is best known for its function as a bacterial second messenger and its roles in bacterial biofilm formation and activation of eukaryotic STING signaling. A recent study published in Nature Chemical Biology demonstrates that cyclic di-AMP is produced in eukaryotic cells as well and has roles in toll-like receptor 9 (TLR9) signaling and NLRP3 inflammasome activation.

Using Cayman's Cyclic di-AMP ELISA Kit to measure cyclic di-AMP levels, the authors of this study discovered that the adenylate cyclase ADCY7 functions downstream of activated TLR9 to synthesize cyclic di-AMP in eukaryotic cells. Cyclic di-AMP then binds to NLRP3 to promote assembly and activation of the inflammasome. This work suggests that synthesis of cyclic di-AMP by ADCY7 may provide a target for regulating the inflammatory response in sterile inflammation.

Read the Article Liu, Q., Tang, Z., Qian, Y., et al. Eukaryotic ADCY7 catalyzes the production of c-di-AMP to activate the NLRP3 inflammasome. Nat. Chem. Biol. 21(8), 1283-1291 (2025)

The Cyclic di-AMP ELISA Kit used in this study is part of Cayman's dedicated toolkit to study CDNs in both eukaryotes and prokaryotes, including additional ELISA kits for cyclic di-GMP, 2'3'-cGAMP, 3'3'-cGAMP, and 3'2'-cGAMP. Our ELISA kits enable sensitive, accurate quantification of CDNs in a variety of sample types.

We also offer a variety of additional tools to study STING signaling, which many CDNs are known to activate, as well as research tools for TLR signaling and the NLRP3 inflammasome.

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