A positive control for COX-2 antibodies
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Mouse Macrophage Microsomal Immunoblot Control

Item No. 10004910

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Mouse Macrophage Microsomes Protein
Formulation (Request formulation change)
500 µg mouse macrophage microsomal protein
Storage Buffer
Laemmli Buffer (2% SDS, 10% glycerol, 0.70 M Tris-HCl, pH 6.8, and 1.5% 2-mercaptoethanol)
Applications
WB
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    Product Description

    Cyclooxygenase 2 (COX-2) is a 72 kDa protein that has been cloned from a variety of species including human, mouse, rat, and ovine.1,2,3,4,5 This preparation of COX-2 microsomes was prepared by solubilization and dilution of the 100,000 x g pellet from the lysate of cytokine-stimulated mouse macrophage cultures. Cayman’s preparation of mouse macrophage microsomes is a ready-to-use sample for SDS-PAGE. As such, it is an excellent positive control for the following Cayman Chemical polyclonal antibodies: Item Nos. 160106, 160126, 160116, and 101750.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Smith, W.L., Garavito, R.M., and DeWitt, D.L. Prostaglandin endoperoxide H synthases (cyclooxygenases)-1 and -2. The Journal of Biological Chemisty 271(52), 33157-33160 (1996).

    2. Hla, T., and Neilson, K. Human cyclooxygenase-2 cDNA. Proc. Natl. Acad. Sci. USA 89(16), 7384-7388 (1992).

    3. Kujubu, D.A., Fletcher, B.S., Varnum, B.C., et al. TIS10, a phorbol ester tumor promoter-inducible mRNA from Swiss 3T3 cells, encodes a novel prostaglandin synthase/cyclooxygenase homologue. The Journal of Biological Chemisty 266(20), 12866-12872 (1991).

    4. Yamagata, K., Andreasson, K.I., Kaufmann, W.E., et al. Expression of a mitogen-inducible cyclooxygenase in brain neurons: Regulation by synaptic activity and glucocorticoids. Neuron 11(2), 371-386 (1993).

    5. Zhang, V., O'Sullivan, M., Hussain, H., et al. Molecular cloning, functional expression, and selective regulation of ovine prostaglandin H synthase-2. Biochem. Biophys. Res. Commun. 227(2), 499-506 (1996).