For immunochemical detection of COX-2
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COX-2 (mouse) Polyclonal Antiserum

Item No. 160116

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Cyclooxygenase 2
  • Prostaglandin H Synthase 2 (PGHS-2)
Immunogen
Peptide from the C-terminal region of mouse COX-2
MW
70 kDa
Formulation (Request formulation change)
100 µl of lyophilized antiserum
Storage Buffer
Polyclonal antiserum when reconstituted in 100 µl of double distilled water
Host
Rabbit
Applications
IF, IHC, WB
Cross Reactivity
(-) COX-1(+) COX-2
Species Reactivity
(+) Human(+) Mouse(+) Ovine
UniProt Accession №
Q05769
Origin
Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    1 ea (100 µl)

    $347

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    Product Description

    Cyclooxygenase 2 (COX-2) is a bifunctional enzyme that exhibits both COX and peroxidase activities and catalyzes the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins.1,2 The COX component converts arachidonic acid to the hydroperoxy endoperoxide prostaglandin G2 (PGG2; Item No. 17010), and the peroxidase component reduces the endoperoxide to the corresponding alcohol PGH2 (Item No. 17020). COX2 expression is induced by a variety of stimuli, including phorbol esters, LPS, and cytokines and is responsible for the biosynthesis of PGs under acute inflammatory conditions.3,4 Thus, COX-2 has been the focus of attention for nonsteroidal anti-inflammatory drug (NSAID) development. Cayman’s COX-2 (mouse) Polyclonal Antiserum can be used for immunofluorescence (IF), immunohistochemistry (IHC), and Western blot (WB) applications. The antiserum recognizes a unique C-terminal region of COX-2 that is not present in COX-1, specifically detecting COX-2 at 70 kDa from human, mouse, and ovine samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Nugteren, D.H., and Hazelhof, E. Isolation and properties of intermediates in prostaglandin biosynthesis. Biochim. Biophys. Acta 326(3), 448-461 (1973).

    2. Hamberg, M., and Samuelsson, B. Detection and isolation of an endoperoxide intermediate in prostaglandin biosynthesis. Proc. Natl. Acad. Sci. USA 70(3), 899-903 (1973).

    3. Kang, Y.-J., Mbonye, U.R., DeLong, C.J., et al. Regulation of intracellular cyclooxygenase levels by gene transcription and protein degradation. Prog. Lipid Res. 46(2), 108-125 (2007).

    4. Blobaum, A.L., and Marnett, L.J. Structural and functional basis of cyclooxygenase inhibition. J. Med. Chem. 50(7), 1425-1441 (2007).

    Product Citations

    Petzold, G.C., Albeanu, D.F., Sato, T.F., et al. Coupling of neural activity to blood flow in olfactory glomeruli is mediated by astrocytic pathways. Neuron. 58(6), 897-910 (2008).

    Kim, D.W., Chi, Y.S., Son, K.H., et al. Effects of sophoraflavanone G, a prenylated flavonoid from Sophora flavescens, on cyclooxygenase-2 and in vivo inflammatory response. Arch. Pharm. Res. 25(3), 329-335 (2002).

    Nantel, F., Meadows, E., Denis, D., et al. Immunolocalization of cyclooxygenase-2 in the macula densa of human elderly. FEBS Lett. 457(3), 475-477 (1999).