For immunochemical detection of COX-2
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COX-2 (mouse) Polyclonal Antibody (aa 570-598)

Item No. 160106

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Cyclooxygenase 2
  • PGHS-2
  • Prostaglandin H Synthase 2
Immunogen
Synthetic peptide from the C-terminal region of mouse/rat COX-2
MW
72 kDa
Formulation (Request formulation change)
500 µl peptide affinity-purified polyclonal antibody
Storage Buffer
PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide
Host
Rabbit
Applications
ICC, IF, IHC, WB
Cross Reactivity
(-) COX-1
Species Reactivity
(+) Human(+) Guinea pig(+) Monkey(+) Mouse(+) Ovine
Origin
Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    1 ea (500 µl)

    $347

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    Product Description

    Cyclooxygenase 2 (COX-2) is a bifunctional enzyme that exhibits both COX and peroxidase activities and catalyzes the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins.1,2 The COX component converts arachidonic acid to the hydroperoxy endoperoxide prostaglandin G2 (PGG2; Item No. 17010), and the peroxidase component reduces the endoperoxide to the corresponding alcohol PGH2 (Item No. 17020). COX2 expression is induced by a variety of stimuli, including phorbol esters, LPS, and cytokines and is responsible for the biosynthesis of PGs under acute inflammatory conditions.3,4 Thus, COX-2 has been the focus of attention for nonsteroidal anti-inflammatory drug (NSAID) development. Cayman's COX-2 (mouse) Polyclonal Antibody (aa 570-598) can be used for immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), and Western blot (WB) applications. The antibody recognizes a unique C-terminal region of COX-2 that is not present in COX-1, specifically detecting COX-2 at 72 kDa from human, guinea pig, monkey, mouse, and ovine samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Nugteren, D.H., and Hazelhof, E. Isolation and properties of intermediates in prostaglandin biosynthesis. Biochim. Biophys. Acta 326(3), 448-461 (1973).

    2. Hamberg, M., and Samuelsson, B. Detection and isolation of an endoperoxide intermediate in prostaglandin biosynthesis. Proc. Natl. Acad. Sci. USA 70(3), 899-903 (1973).

    3. Kang, J.G., and Park, C.-Y. Anti-obesity drugs: A review about their effects and safety. Diabetes Metab. J. 36(1), 13-25 (2012).

    4. Blobaum, A.L., and Marnett, L.J. Structural and functional basis of cyclooxygenase inhibition. J. Med. Chem. 50(7), 1425-1441 (2007).

    Product Citations

    Kelleher, A.M., Milano-Foster, J., Behura, S.K., et al. Uterine glands coordinate on-time embryo implantation and impact endometrial decidualization for pregnancy success. Nat. Commun. 9(1), 2435 (2018).

    Corwin, C., Nikolopoulou, A., Pan, A.L., et al. Prostaglandin D2/J2 signaling pathway in a rat model of neuroinflammation displaying progressive parkinsonian-like pathology: Potential novel therapeutic targets. J. Neuroinflammation 15(1), 272 (2018).

    Pennock, N.D., Martinson, H.A., Guo, Q., et al. Ibuprofen supports macrophage differentiation, T cell recruitment, and tumor suppression in a model of postpartum breast cancer. J. Immunother. Cancer 6(1), 98 (2018).

    Hull, M.A., Cuthbert, R.J., Ko, C.W.S., et al. Paracrine cyclooxygenase-2 activity by macrophages drives colorectal adenoma progression in the Apc Min/+ mouse model of intestinal tumorigenesis. Sci. Rep. 7(1), 6074 (2017).

    Shojo, H., Borlongan, C.V., and Mabuchi, T. Genetic and histological alterations reveal key role of prostaglandin synthase and cyclooxygenase 1 and 2 in traumatic brain Injury-induced neuroinflammation in the cerebral cortex of rats exposed to moderate fluid percussion injury. Cell Transplant 26(7), 1301-1313 (2017).

    Gouveia-Figueira, S., Karlsson, J., Deplano, A., et al. Characterisation of (R)-2-(2-fluorobiphenyl-4-yl)-N-(3-methylpyridin-2-yl)propanamide as a dual fatty acid amide hydrolase: Cyclooxygenase inhibitor. PLoS One 10(9), e0139212 (2015).

    Zhang, W.C., Zheng, X.J., Du, L.J., et al. High salt primes a specific activation state of macrophages, M(Na). Cell Res. 25(8), 893-910 (2015).

    Dong, Z.W., Chen, J., Ruan, Y.C., et al. CFTR-regulated MAPK/NF-κB signaling in pulmonary inflammation in thermal inhalation injury. Sci. Rep. 5, 15946 (2015).

    Zaiss, A.K., Zuber, J., Chu, C., et al. Reversible suppression of cyclooxygenase 2 (COX-2) expression in vivo by inducible RNA interference. PLoS One 9(7), e101263 (2014).

    Roulis, M., Nikolaou, C., Kotsaki, E., et al. Intestinal myofibroblast-specific Tpl2-Cox-2-PGE2 pathway links innate sensing to epithelial homeostasis. Proc. Natl. Acad. Sci. USA 111(43), E4658-E4667 (2014).

    Lei, B., Mace, B., Dawson, H.N., et al. Anti-inflammatory effects of progesterone in lipopolysaccharide-stimulated BV-2 microglia. Plos One 9(7), e103969 (2014).

    Fornetti, J., Jindal, S., Middleton, K.A., et al. Physiological COX-2 expression in breast epithelium associates with COX-2 levels in ductal carcinoma in situ and invasive breast cancer in young women. Am. J. Pathol. 184(4), 1219-1229 (2014).

    Sanchez, A., Tripathy, D., Yin, X., et al. Sunitinib enhances neuronal survival in vitro via NF-κB-mediated signaling and expression of cyclooxygenase-2 and inducible nitric oxide synthase. J. Neuroinflammation 10, 93 (2013).

    Sun, Y., Jia, Z., Liu, G., et al. PPARγ agonist rosiglitazone suppresses renal mPGES-1/PGE2 pathway in db/db mice. PPAR Res. 2013:e612971, (2013).

    Kim, C.W., Vo, M.-T., Kim, H.K., et al. Ectopic over-expression of tristetraprolin in human cancer cells promotes biogenesis of let-7 by down-regulation of Lin28. Nucleic Acids Res. 40(9), 3856-3869 (2012).

    Wu, T., Wu, H., Wang, J., et al. Expression and cellular localization of cyclooxygenases and prostaglandin E synthases in the hemorrhagic brain. J. Neuroinflammation 8, 22 (2011).

    Dougherty, U., Mustafi, R., Wang, Y., et al. American ginseng suppresses Western diet-promoted tumorigenesis in model of inflammation-associated colon cancer: role of EGFR. BMC Complement. Altern. Med. 11, 111 (2011).

    Toller, I.M., Hitzler, I., Sayi, A., et al. Prostaglandin E2 prevents Helicobacter-induced gastric preneoplasia and facilitates persistent infection in a mouse model. Gastroenterology 138(4), 1467 (2010).

    Melnick, M., Mocarski, E.S., Abichaker, G., et al. Cytomegalovirus-induced embryopathology: Mouse submandibular salivary gland epithelial-mesenchymal ontogeny as a model. BMC Dev. Biol. 6, 42 (2006).

    Shattuck-Brandt, R.L., Varilek, G.W., Radhika, A., et al. Cyclooxygenase 2 expression is increased in the stroma of colon carcinomas from IL-10(-/-) mice. Gastroenterology 118(2), 337-345 (2000).

    Kömhoff, M., Guan, Y., Shappell, H.W., et al. Enhanced expression of cyclooxygenase-2 in high grade human transitional cell bladder carcinomas. Am. J. Pathol. 157(1), 29-35 (2000).

    Tessner, T.G., Cohn, S.M., Schloemann, S., et al. Prostaglandins prevent decreased epithelial cell proliferation associated with dextran sodium sulfate injury in mice. Gastroenterology 115(4), 874-882 (1998).