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Displaying 1 - 9 of 9 Results

​Cell Cycle and DNA Damage

Brochures

​The cell cycle is a tightly regulated process of cell growth, DNA replication, and cell division. Progression through the phases of the cell cycle is controlled by cyclins in association with cyclin-dependent kinases (CDKs). Throughout this process, cells encounter several checkpoints designed to detect and correct issues such as DNA damage or improper spindle formation before allowing continuation of the cell cycle. Dysregulation of the cell cycle and checkpoint mechanisms can lead to aberrant proliferation and genomic instability and is often observed in cancer cells.

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Cell Cycle and DNA Damage

​Kinase Inhibitors

Brochures

The signal transduction pathways responsible for virtually every cell function in the body are built on a network of kinases. Because kinases are so acutely involved in the regulation of biological processes, the field of drug discovery endeavors to validate novel kinase targets for therapeutic intervention as well as to develop and improve target selectivity of modulatory small molecules. Use the signal transduction cascades inside this brochure to explore Cayman's broad selection of inhibitors targeting specific nodes of the major kinase signaling pathways. 
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Kinase Inhibitors

​High-Throughput Flow Cytometric Screening of a Kinase Inhibitor Library for Apoptosis Induction

Application notes

​Key Information

  • Cayman’s multiparametric Early Apoptosis Detection Assay Kit used with Miltenyi Biotec’s MACSQuant® X Flow Cytometer creates a powerful, efficient, and exquisitely detailed screening tool.
  • Using this approach to screen a library of kinase inhibitors for cytotoxicity, apoptosis inducers were distinguished from necrosis-causing or mitochondrial-uncoupling compounds.
  • This simple approach can be adapted to many other probes for cellular function to help determine the effects of potential therapeutic compounds during the drug development process.


To cite this application note: Rumble, J.M., Hoffman, D.L. High-Throughput Flow Cytometric Screening of a Kinase Inhibitor Library for Apoptosis Induction. Application Note, Cayman Chemical (2020).

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Screening a Kinase Inhibitor Library for Mitochondrial Dysfunction

Scientific posters

​Mitochondrial toxicity from compound exposure can be pinpointed with coordinated OCR/ECAR analysis and mechanism of action assays.

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To cite this poster: Hoffman, D.L. Screening a kinase inhibitor library for mitochondrial dysfunction. Poster presented at: MichBio's 2019 Cutting Edge / Drug Discovery & Development in Michigan Symposium; November 12, 2019; Ann Arbor, MI.


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Flow Cytometric Screening of a Kinase Inhibitor Library for Apoptosis Induction

Scientific posters

​Multiparametric screening of kinase inhibitor cytotoxicity distinguishes multiple modes of cell death.

Do you have a question or comment for the presenter? Let us know.

To cite this poster: Rumble, J.M. and Hoffman, D.L. Flow cytometric screening of a kinase inhibitor library for apoptosis induction. Poster presented at: MichBio's 2019 Cutting Edge / Drug Discovery & Development in Michigan Symposium; November 12, 2019; Ann Arbor, MI.

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​A New, Efficient Synthesis of ω-NBD Sphingosine

Scientific posters

​ω-NBD sphingosine is a biologically active derivative of sphingosine that is tagged with a fluorescent nitrobenzoxadiazole (NBD) group. Sphingosine (d18:1) (Cayman Item No. 10007907) is an important precursor to sphingosine-1-phosphate. The two enzymes that are responsible for this process are the sphingosine kinases SPHK1 and SPHK2. The fluorescently tagged ω-NBD sphingosine (Cayman Item No. 25348) allows researchers to monitor the activity of these kinase enzymes in real time. ω-NBD sphingosine has been previously described in literature, and the former method of synthesis was thirteen steps long. We have developed a more efficient method of synthesis that is only six steps long. 

Do you have a question or comment for the presenter? Let us know.

To cite this poster: Hyder, S. and Uzieblo, A. A new, efficient synthesis of ω-NBD sphingosine. Poster presented at: Bioactive Lipids in Cancer, Inflammation, and Related Diseases 16th International Conference; October 20-23, 2019; St. Petersburg, FL.


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Examining the FP receptor-triggered CREB activation in cell-based assays

Scientific posters

The PGF2α receptor (FP) was initially characterized as a Gq-coupled receptor, which leads to an increase in inositol triphosphate/diacylglycerol, intracellular calcium mobilization, and protein kinase C (PKC) activation. Interestingly, PGF2α has also been suggested to induce CREB- regulated genes in human amniotic fibroblasts and tracheobronchial epithelial cells through PKC activity. The activation of CREB by the FP receptor has been reproduced in this study by co-transfecting the FP receptor with a CRE-SEAP reporter into HEK293T cells using reverse transfection.

To cite this poster: Ho, C.S., Patel, C., Saepoo, B., et al. Examining the FP receptor-triggered CREB activation in cell-based assays. Poster presented at: 2018 ASCB|EMBO Annual Meeting; December 8-12, 2018; San Diego, CA.
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​Autophagy and Apoptosis

Brochures

Autophagy and apoptosis regulate cell survival and death in response to particular stress signals. Cell fate decisions are conferred through the complex crosstalk of a core regulatory signaling network involving cytoplasmic calcium, AMP-activated protein kinase, p53, Bcl-2, and caspases.
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​Heat Shock Proteins

Brochures

Heat shock proteins (Hsps) play a central role in cellular homeostasis, facilitating protein synthesis and folding throughout the cell as well as participating in protein trafficking, protein degradation, and the regulation of various kinases and transcription factors. 
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Displaying 1 - 9 of 9 Results