An Analytical Reference Material
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  • This product is qualified as a Reference Material that has been manufactured and tested to meet ISO/IEC 17025 and ISO 17034 international standards
  • This product is intended to be used as an analytical reference standard
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Isotonitazene

Item No. 27255

Restricted Product (Schedule I)
Product Insert (PDF)GC-MS (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N,N-diethyl-2-[[4-(1-methylethoxy)phenyl]methyl]-5-nitro-1H-benzimidazole-1-ethanamine
CAS Number
14188-81-9
Molecular Formula
C23H30N4O3
Formula Weight
410.5
Purity
≥98%
Formulation
A neat solid
SMILES
CCN(CC)CCN1C(CC2=CC=C(OC(C)C)C=C2)=NC3=CC([N+]([O-])=O)=CC=C31
InChi Code
InChI=1S/C23H30N4O3/c1-5-25(6-2)13-14-26-22-12-9-19(27(28)29)16-21(22)24-23(26)15-18-7-10-20(11-8-18)30-17(3)4/h7-12,16-17H,5-6,13-15H2,1-4H3
InChi Key
OIOQREYBGDAYGT-UHFFFAOYSA-N
Regulatory Information
DEA Schedule
I
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    Product Description

    Isotonitazene (Item No. 27255) is an analytical reference material categorized as an opioid.1,2,3 Isotonitazene has analgesic effects in mice.4 It has been associated with overdoses.5 Isotonitazene is regulated as a Schedule I compound in the United States. This product is intended for research and forensic applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Casy, A.F., and Wright, J. Ionisation constants and partition coefficients of some analgesically active 2-benzylbenzimidazole derivatives and related compounds. J. Pharm. Pharmacol. 18(10), 677-683 (1966).

    2. Blanckaert, P., Cannaert, A., Van Uytfanghe, K., et al. Report on a novel emerging class of highly potent benzimidazole NPS opioids: Chemical and in vitro functional characterization of isotonitazene. Drug Test. Anal. 12(4), 422-430 (2019).

    3. Vandeputte, M.M., Van Uytfanghe, K., Layle, N.K., et al. Synthesis, chemical characterization, and µ-opioid receptor activity assessment of the emerging group of "nitazene" 2-benzylbenzimidazole synthetic opioids. ACS Chem. Neurosci. 12(7), 1241-1251 (2021).

    4. Ujváry, I., Christie, R., Evans-Brown, M., et al. DARK classics in chemical neuroscience: Etonitazene and related benzimidazoles. ACS Chem. Neurosci. 12(7), 1072-1092 (2021).

    5. Shover, C.L., Falasinnu, T.O., Freedman, R.B., et al. Emerging characteristics of isotonitazene-involved overdose deaths: A case-control study. J. Addict. Med. 15(5), 429-431 (2021).

    Product Citations

    Hataoka, K., Hojo, M., Nomura, S., et al. Evaluation of rewarding effects of nitazene analogs: results from conditioned place preference tests and in vivo microdialysis experiments in mice. J. Toxicol. Sci. 50(1), 33-43 (2025).

    Tsai, M.-H.M., Chen, L., Baumann, M.H., et al. In Vitro Functional Profiling of Fentanyl and Nitazene Analogs at the μ-Opioid Receptor Reveals High Efficacy for Gi Protein Signaling. ACS Chem. Neurosci. 15(4), 854-867 (2024).

    Taoussi, O., Berardinelli, D., Zaami, S., et al. Human metabolism of four synthetic benzimidazole opioids: Isotonitazene, metonitazene, etodesnitazene, and metodesnitazene. Arch. Toxicol. (2024).

    Vandeputte, M.M., Glatfelter, G.C., Walther, D., et al. Characterization of novel nitazene recreational drugs: Insights into their risk potential from in vitro µ-opioid receptor assays and in vivo behavioral studies in mice. Pharmacol. Res. 210, 107503 (2024).

    Vandeputte, M.M., Van Uytfanghe, K., Layle, N.K., et al. Synthesis, chemical characterization, and µ-opioid receptor activity assessment of the emerging group of "nitazene" 2-benzylbenzimidazole synthetic opioids. ACS Chem. Neurosci. 12(7), 1241-1251 (2021).

    Vandeputte, M.M., Cannaert, A., and Stove, C.P. In vitro functional characterization of a panel of non‑fentanyl opioid new psychoactive substances. Arch. Toxicol. 94(11), 3819-3830 (2020).

    Krotulski, A.J., Papsun, D.M., Kacinko, S.L., et al. Isotonitazene quantitation and metabolite discovery in authentic forensic casework. J. Anal. Toxicol. 44(6), 521-530 (2020).

    Blanckaert, P., Cannaert, A., Van Uytfanghe, K., et al. Report on a novel emerging class of highly potent benzimidazole NPS opioids: Chemical and in vitro functional characterization of isotonitazene. Drug Test. Anal. 12(4), 422-430 (2019).