An mRNA for the expression of Lifeact-mTurquoise2
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Lifeact-mTurquoise2 Probe mRNA (Cap-1; m1Ψ)

Item No. 43684

Product Insert (PDF)
Technical Information
Synonyms
  • Lifeact-mTurquoise2 Probe mRNA (Cap-1; N1-Methylpseudouridine)
Emission
474 nm
Excitation
434 nm
Formulation
1 mM Sodium citrate, pH 6.4
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    Product Description

    Lifeact-mTurquoise2 probe mRNA (Cap-1; m1Ψ) encodes a fusion protein composed of a Lifeact peptide, which selectively binds to actin fibers, and mTurquoise2, a cyan fluorescent protein that displays excitation and emission maxima of 434 and 474 nm, respectively.1 It is capped using a co-transcriptional capping method, resulting in the naturally occurring Cap-1 structure with high capping efficiency. Lifeact-mTurquoise2 probe mRNA is also polyadenylated and modified with N1-methylpseudouridine (m1Ψ) to reduce the host cell immune response and enhance mRNA stability. It can be used in the study of actin structure and function in live cells. Encapsulation of Lifeact-mTurquoise2 probe mRNA (Cap-1; m1Ψ) in lipid nanoparticles (LNPs) can be used for mRNA delivery and expression of Lifeact-mTurquoise2.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Wang, J., Cao, Z., Zhang, Q., et almRNA-encoded fluorescent proteins enable superior organelle labeling for live cell imaging. FASEB J. 38(23), e70244 (2024).